数字PCR在食源性致病微生物检测中的 应用研究进展

Research progress on the application of digital PCR in detection of foodborne pathogenic microorganisms

中文关键词:  数字PCR  微滴式数字PCR  芯片式数字PCR  食源性致病微生物

英文关键词:digital PCR  droplet digital PCR  chip digital PCR  foodborne pathogenic microorganisms

中文摘要:

      大多数食源性疾病由食源性致病微生物引发, 研究和建立食源性致病微生物的快速有效检测方法对于食品安全风险监控及保障人们的身体健康具有重要意义。相对于传统PCR, 数字PCR具有较好的准确度和重现性, 可实现绝对定量分析, 为快速准确地进行食品安全检测提供了一种崭新的技术平台。本文主要介绍了数字PCR中微滴式数字PCR和芯片式数字PCR的基本原理、种类、应用及其研究进展, 深入探讨了数字PCR技术在沙门氏菌、大肠杆菌O157:H7、单核细胞增生李斯特氏菌、阴沟杆菌和金黄色葡萄球菌等食源性致病微生物检测中的应用。数字PCR技术目前在转基因成分和动物源性成分定量检测中都得到了较好的应用, 在食源性致病微生物中的应用技术还有待更好的发展。

英文摘要:

      Foodborne diseases are mostly caused by foodborne pathogens, so it is very important to establish effective detection methods of foodborne pathogenic microorganisms for food safety risk monitoring and people's health ensuring. Compared with the traditional PCR, digital PCR has good accuracy and reproducibility, which has provided a new platform for rapid detection of food safety. This article mainly summarized the basic principles, varieties, application and research progress of digital PCR technology including droplet digital PCR and chip digital PCR. The applications of digital PCR technology in detection of Salmonella, Escherichia coli O157:H7, Listeria monocytogenes, Enterobacter cloacae, Staphylococcus aureus and other foodborne pathogenic microorganisms were also discussed in depth. The technology digital PCR has gotten good applications in quantitative detection of genetically modified organisms and animal derived ingredients, but was remained to be better in the application of foodborne pathogenic microorganisms.