改良琼脂扩散法鉴定麦卢卡蜂蜜

Identification of manuka honey by a modified agar well diffusion method

中文关键词:  麦卢卡蜂蜜  非过氧化氢活性  独麦素  琼脂扩散法  金黄色葡萄球菌

英文关键词:manuka honey  non-peroxide activity  Unique Manuka Factor  agar well diffusion method  Staphylococcus aureus

中文摘要:

      目的 改良Allen等提出的测定麦卢卡蜂蜜非过氧化氢抑菌活性的琼脂扩散法。方法 参照Allen等提出的琼脂扩散法, 研究以下3个方面对琼脂扩散法的影响: 使用恒温箱或在杂交炉中混匀蜂蜜水溶液, 使用不同代的金黄色葡萄球菌菌株和培养基的倾斜程度。结果 麦卢卡蜂蜜样品溶液使用恒温箱和杂交炉静置法混匀, 测得样品的独麦素(Unique Manuka Factor, )UMF值没有显著差异; 随着金黄色葡萄球菌传代数的增加, 样品的UMF值也没有显著差异; 培养基倾斜后同一麦卢卡蜂蜜位于中间样孔的样品UMF值明显高于边际样孔。结论 杂交炉混匀蜂蜜的方法操作简单, 更有利于麦卢卡蜂蜜非过氧化氢活性的检测, 25代以内的任何1代菌株均可用于测定麦卢卡蜂蜜样品的非过氧化氢活性, 培养基倾倒时要尽量避免过度倾斜。

英文摘要:

      Objective To improve the agar well diffusion method which be used to determine the non-peroxide activity (NPA) of manuka honey proposed by Allen et al. Methods The effects of the following 3 aspects on agar well diffusion method were studied referring to the method proposed by Allen et al.: mixed honey samples by thermostat or hybridization oven, the use of different generations of Staphylococcus aureus strains and inclination of medium. Results The UMF value (Unique Manuka Factor) of manuka honey solutions which mixed in thermostat and hybridization oven had no significant difference. With the increase of passages of S. aureus, the UMF value of samples had no significant difference. And the UMF value of samples located in the middle sample holes were significantly higher than that in the marginal holes on the same inclined medium. Conclusion The mixture of honey samples in hybridization oven is more convenient to operate, and be advantageous to determine non-peroxide activity of manuka honey. Any S. aureus within 25 generations can be used to determine non-peroxide activity of manuka honey samples. The excessive inclination of medium should be avoided when it is dumped.