粮油作物及饲料转基因成分检测能力验证 结果分析

Analysis of proficiency test for detecting genetically modified component in crops and feed

中文关键词:  转基因成分  能力验证  结果  影响因素

英文关键词:genetically modified components  proficiency testing  result  influencing factors

中文摘要:

      目的 通过参加转基因检测能力验证计划, 对实验室转基因成分检测技术能力进行有效确认。方法 针对能力验证组织方的要求, 依据相应国家标准、农业部公告及出入境检验检疫行业标准, 对水稻、玉米、玉米酒糟粕、大豆和油菜籽共10份样品进行转基因成分定性检测和品系鉴定。结果 所参加5项10份样品的能力验证均获得满意结果。结论 转基因成分检测能力验证结果受检测方法的选择、DNA提取的效率与质量、实验过程的质量控制等因素影响较大。

英文摘要:

      Objective To make valid confirmation of the technical ability for genetically modified component detection in our laboratory by participating in the proficiency tests for detecting genetically modified components. Methods According to proficiency test organizer’s requirements, genetically modified components of 10 samples including rice, corn, corn distillers dried grains (DDG), soybean and rapeseed were qualitative detected or event identified, in accordance with the corresponding GB/T standards, announcements of the ministry of agriculture PRC and SN/T standards. Results All the 5 proficiency tests and 10 samples were shown satisfactory results. Conclusion Results of proficiency tests are greatly affected by the choice of detection method, the extraction efficiency and quality of DNA, the quality control of experiment process etc.