食品中金黄色葡萄球菌定量检测能力验证 结果与分析

Proficiency testing results and analysis of quantitative detection of Staphylococcus aureus in food

中文关键词:  金黄色葡萄球菌  定量  能力验证  鉴定  分型

英文关键词:Staphylococcus aureus  quantitative  proficiency testing  identification  ribotyping

中文摘要:

      目的 提升实验室对食品中金黄色葡萄球菌的检测能力。方法 按照能力验证作业指导书规定的方法要求进行检测, 并采用全自动微生物基因指纹鉴定系统(RiboPrinter?)对盲样中添加的菌株进行鉴定与分型。结果 编号为CODE 1~9的样品均检出金黄色葡萄球菌, 编号为CODE 10的样品未检出金黄色葡萄球菌。阳性样品的Z值结果均＜2; 阴性样品的结果为＜10 CFU/g, 10个样品测试均取得满意结果。结论 通过开展能力验证工作可以较好地提高实验室检测能力。

英文摘要:

      Objective To improve the detection capacity of Staphylococcus aureus in food. Methods Detection was followed the instruction of the proficiency testing, and RiboPrinter? was used for identification and ribotyping of the bacteria added into the blind sample. Results Staphylococcus aureus was detected in CODE 1~9 and there was no Staphylococcus aureus in CODE 10. The Z scores of the positive samples were all less than 2 and the result of the negative sample was less than 10 CFU/g. The test results of 10 samples were satisfied. Conclusion The laboratory detection capability has been improved by implementing proficiency testing.