脂溶性化妆品原料中溴代十六烷检测方法的研究

Determination of 1-bromohexadecane in liposoluble cosmetics ingredients

中文关键词:  化妆品原料  溴代十六烷  气相色谱法  电子捕获检测器  卤代烃

英文关键词:cosmetics ingredients  1-bromohexadecane  gas chromatography  electron capture detector  halogenated hydrocarbon

中文摘要:

      目的 建立配备电子捕获检测器的气相色谱法(gas chromatography, GC)测定脂溶性化妆品原料中溴代十六烷杂质残留的方法。方法 样品经甲醇超声溶解, 加水滤去原料主成分后, 经水饱和的正己烷萃取, 加硫酸净化后进行GC测定。GC采用DB-1色谱柱分离, 以外标法定量。结果 溴代十六烷在0.0951~1.902 μg/mL浓度范围内线性关系良好, 相关系数为0.9999; 方法检出限为0.19 μg/g, 在低中高3浓度加标水平下, 平均回收率为87.9%~92.3%, 相对标准偏差为2.5%(n=2)。结论 本方法准确度高、重现性好, 可适用于脂溶性化妆品原料中溴代十六烷残留量的检测。

英文摘要:

      Objective To establish a method for determination of 1-bromohexadecane in liposoluble cosmetics ingredients by gas chromatography (GC) with electron capture detector. Methods The sample was processed by ultrasonic dissolving with methanol, added water in the solution and filtered out the principal component of ingredients, extracted by hexane which was saturated by water and purified with sulphuric acid, and then analyzed by GC. The target compound was separated by DB-1 chromatographic column and qualified by external standard. Results 1-bromohexadecane had a good linear relationship in the range of 0.0951~1.902 μg/mL with the correlation coefficient of 0.9999. The limit of detection (LOD) of the method was 0.19 μg/g. The average recoveries of the method were ranged in 87.9%~92.3% and RSD were 2.5% (n=2). Conclusion This method has high accuracy and good reproducibility, which is suitable for the determination of 1-bromohexadecane in liposoluble cosmetics ingredients.