高效液相色谱-串联质谱法测定中国劲酒中 甜菜碱含量

Determination of betaine in Jin liquor by high performance liquid chromatography-tandem mass spectrometry

中文关键词:  中国劲酒  甜菜碱  高效液相色谱-串联质谱法

英文关键词:Jin liquor  betaine  high performance liquid chromatography-tandem mass spectrometry

中文摘要:

      目的 建立高效液相色谱-串联质谱法测定中国劲酒中甜菜碱。方法 采用Agilent SB-C18色谱柱, 以0.1%甲酸-甲醇溶液(85:15, V:V)为流动相, 流速为0.2 mL/min。质谱采用电喷雾正电离源模式(electronic spray ion, ESI+)和多反应监测模式(multiple reactions monitoring, MRM)监测, 监测离子m/z分别为118.2/53.2和118.2/42.3, 以外标法定量。结果 甜菜碱在3.99~39.9 μg/g范围内具有良好的线性关系, 相关系数大于0.999, 检出限为0.1 μg/g, 平均回收率为99.5%, 相对标准偏差为0.46%(n=9)。结论 本法简便易行、结果准确、重现性好, 可适用于中国劲酒中甜菜碱的含量测定。

英文摘要:

      Objective To establish a method for determination of betaine in Jin liquor by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods Samples were separated by an Agilent SB-C18 column (100 mm×2.1 mm,1.8 μm) using 0.1% methanoic acid–methanol (85:15, V:V) as mobile phase at the flow rate of 0.2 mL/min. Electronic spray ion (ESI+) and multiple reactions monitoring (MRM) mode were used in mass with the m/z of ionization source at 118.2/58.2 and 118.2/42.3, and the external standard method was used for quantification. Results Betaine had a good linear relationship in the range of 3.99~39.9 μg/g (r>0.999), the limit of detection was 0.1 μg/g, the average recovery was 99.5%, and RSD was 0.46% (n=9). Conclusion The method is simple, rapid, accurate and repeatable, which can be used for the determination of betaine in Jin liquor.