马尾松花粉提取物对小鼠前列腺肥大的预防作用

Preventive effect of Pinus massoniana pollen extract on benign prostate hyperplasia in mice

中文关键词:  马尾松花粉  提取物  良性前列腺肥大  炎症反应  氧化应激

英文关键词:masson pine pollen  extract  benign prostatic hyperplasia  inflammatory response  oxidative stress

中文摘要:

      目的 探究马尾松花粉提取物对小鼠前列腺肥大(benign prostatic hyperplasia, BPH)的影响。方法 雄性昆明小鼠随机分为6组, 分别为空白对照组、BPH模型组、20 mg/kg?d低剂量、40 mg/kg?d中剂量、80 mg/kg?d高剂量马尾松花粉提取物组和非那雄胺(0.8 mg/kg?d)组。预先灌胃松花粉提取物2周之后, 在腹腔注射丙酸睾酮建模的同时连续灌胃3种剂量的马尾松花粉提取物或非那雄胺4周。实验结束后, 用试剂盒测定IL-1?、IL-6、TNF-?、DHT、NO、NOS以及PACP的水平; 另外剖取前列腺和脾脏, 测定前列腺指数和脾脏指数。结果 3种剂量的松花粉处理均降低了BPH小鼠的前列腺指数、炎性细胞因子、DHT浓度及PACP、NOS的活性, 并减少了NO的生成。结论 马尾松花粉提取物可以明显降低BPH病理症状及相关生化指标, 对BPH起到一定的保护作用, 其作用机制可能与其抗炎抗氧化活性有关。

英文摘要:

      Objective To explore the influences of masson pine pollen extract on benign prostate hyperplasia (BPH) in mice. Methods Kunming male mice were randomly divided into 6 groups, which were normal control group, BPH model group, 20 mg/kg (low), 40 mg/kg (middle), 80 mg/kg(high) masson pine pollen extract groups and Finasteride group (0.8 mg/kg?d). Mice of 3 pollen groups were intragastrically administrated with pollen extract for 2 weeks in advance. Using testosterone propionate to build the BPH mice model meanwhile pollen extract and finasteride were intragastrically administrated respectively for 4 weeks continuously. At the end of experiment, the prostate gland and spleen were dissected for determination of prostate index (PI) and spleen index(SI), and IL-6, IL-1β, TNF-α, PACP, DHT, NO, NOS and PACP were measured by test kits. Results Three doses of masson pine pollen extract all decreased PI, cytokines, DHT concentration, the activities of PACP and NOS, and reduced the generation of NO. Conclusion Masson pine pollen extract can significantly reduce the pathological symptoms and biochemical parameters of BPH so as to protect BPH in some extent, and the mechanism may be related to the inhibition of inflammation and antioxidant activity.