基于Fe3+催化荧光增强的抗坏血酸检测

The determination of ascorbic acid based on the fluorescence enhancement catalyzed by Fe3+

中文关键词:  抗坏血酸  邻苯二胺  荧光响应

英文关键词:ascorbic acid  o-phenylenediamine  fluorescent response

中文摘要:

      目的 建立了一种基于Fe3+催化的荧光增强的传感平台用于抗坏血酸的灵敏检测。方法 抗坏血酸可以和邻苯二胺反应生成一种具有π键共轭结构的氮杂环的荧光化合物, 从而实现检测抗坏血酸; Fe3+能够加速上述反应的进程。结果 在0.2~1.7 mmol/L浓度范围内, 体系荧光信号和抗坏血酸浓度成线性关系, 相关系数r=0.997, 检测限为0.35 μmol/L。此外, 常见的干扰物不影响抗坏血酸的选择性测定。结论 该方法具有较高的灵敏度, 低廉的成本和简便的操作步骤, 并且易于实现定量分析。

英文摘要:

      Objective To construct a fluorescence sensing platform for sensitive detection of ascorbic acid based on the catalysis of Fe3+. Methods Ascorbic acid can react with o-phenylenediamine to form a N-heterocyclic fluorescent compound with large conjugated p-bond structure, while Fe3+ can accelerate the reaction, which could realize the detection of ascorbic acid successfully. Results The fluorescence value was linearly dependent on the concentration of ascorbic acid in the range of 0.2~1.7 mmol/L with the correlation coefficient of 0.997, and the limit of detection was 0.35 μmol/L. In addition, the common interference had no effect on the selective determination of ascorbic acid. Conclusion The proposed method is sensitive, costless and simple, which can be readily applied to acquire quantitative information.