转基因速测卡的现场应用性能及其可行性研究

Field application and feasibility of genetically modified dipstick

中文关键词:  转基因  检测  速测卡  Bt蛋白

英文关键词:genetically modified organism  detection  dipstick  Bt protein

中文摘要:

      目的 对快速、灵敏、适用于现场检测的转基因速测卡的现场实际应用性能进行评估, 并对其方案设计进行探索性研究。方法 选取国内外3家公司的转基因速测卡对含Cry1Ab/1Ac Bt蛋白的转基因水稻的检测性能(如灵敏度和交叉反应性)进行评估, 并与Real-time PCR 方法同时对5种模拟实际样品进行检测分析。结果 转基因速测卡的灵敏度最低可达0.25%, 且无交叉反应性, 与Real-time PCR法检测模拟实际样品的检测结果一致, 此外, 转基因速测卡具有良好的现场操作性能。结论 转基因速测卡具有良好的实际现场应用性能, 当实际应用检测时, 应根据检测目的与目标蛋白抗原, 设计相应的实验方案, 达到准确检测的目的。因此, 转基因速测卡具有良好的市场推广前景。

英文摘要:

      Objective To evaluate the performance of the field application and design of experimental scheme of rapid, sensitive transgenic dipstick. Methods In this study, the detection performance of the transgenic dipsticks from 3 companies were evaluated with transgenic rice Cry1Ab/Cry1Ac Bt protein, such as sensitivity and cross reactivity, and 5 different samples were detected by the transgenic dipsticks and real time PCR. Results The results showed that the minimum limit of detection was up to 0.25% and there was no cross reactivity. The results of the detection of 5 different samples with the transgenic dipsticks were consistent with the real-time PCR method. In addition, transgenic dipsticks had a good performance in field operation. Conclusion The transgenic dipstick shows a good practical application performance. When used for actual application testing, it should be designed corresponding experimental scheme to achieve accurate detection, according to the testing purpose and target protein antigens. Therefore, the transgenic dipstick will have a good market prospect.