肽核酸探针在微生物快速检测中的应用

Application of peptide nucleic acid probe in rapid detection

中文关键词:  肽核酸  探针  微生物  快速检测

英文关键词:peptide nucleic acid  probe  microorganisms  rapid detection

中文摘要:

      致病性微生物引起的食源性疾病在突发事件数和患者数方面占比最多。如何对食源性致病微生物进行快速、准确的检测, 已成为食品安全应急处理亟待解决的问题。第3代反义核酸-肽核酸(peptide nucleic acid, PNA)探针由于不带电荷的结构特点, 其稳定性、特异性、亲和性和膜通透性均优于DNA探针。近年来PNA在微生物快速检测中得到越来越广泛的应用, 如用于荧光原位杂交、PCR扩增、生物传感器、基因芯片以及Southern印迹等。本文介绍了PNA探针的结构和特点、杂交方式以及PNA探针的设计原则, 对其在微生物快速筛选方面的应用进展进行了综述, 在此基础上分析了PNA探针的优缺点, 并对未来发展趋势进行了展望。

英文摘要:

      Food borne diseases caused by microorganisms are the maximum in numbers of emergency events or patients. How to detect food borne pathogenic microorganisms rapidly and accurately has become an urgent problem to be solved in the food safety emergency handling. The third generation antisense nucleic acid-peptide nucleic acid (PNA) probe was better than DNA probe in the respects of stability, specificity, compatibility and membrane permeability, owing to its characteristic of no electric charge. PNA was widely applied in the rapid detection of microorganisms recently, for instance in PNA-FISH, PCR, biosensor, gene chip and Southern blotting, etc. In this paper, the structure characteristic, pattern of hybridization and PNA probe design principle were introduced, and application of PNA probe in rapid microbial screening were reviewed, also the advantages and disadvantages were analyzed and the future development trend was prospected.