食品中沙门氏菌快速检测技术研究进展

Research progress of rapid detection technique of Salmonella in food

中文关键词:  沙门氏菌  酶联免疫吸附法  聚合酶链反应  环介导等温扩增  基质辅助激光解析电离飞行时间质谱

英文关键词:Salmonella  enzyme-linked immunosorbent assay  polymerase chain reaction  loop-mediated isothermal amplification  matrix assisted laser desorption/ionization time of flight mass spectrometry

中文摘要:

      沙门氏菌的检验在食品微生物检验中具有十分重要的意义。本文主要针对基于免疫学的快速检测方法-酶联免疫吸附法(enzyme-linked immunosorbent assay, ELISA)、基于分子生物学的聚合酶链反应(polymerase chain reaction, PCR)、环介导等温扩增(loop-mediated isothermal amplification, LAMP)和基于蛋白质的基质辅助激光解析电离飞行时间质谱法(matrix assisted laser desorption/ionization time of flight mass spectrometry, MALDI-TOF MS)在食品中沙门氏菌快速检测的应用进行了分析与比较。基于3种不同原理的检测方法各有其优缺点, ELISA方法成本低, 操作简单但检出限高; PCR方法成本较高, 但检测快速、灵敏度高且检出限低; MALDI-TOF MS检出限低但需要完善的细菌库进行比对。因此建议将含内控的荧光定量PCR与LAMP法作为食品中检测沙门氏菌的主要方法。

英文摘要:

      Detection of Salmonella has an important significance in food microbiology inspection. The application of rapid methods for detection of Salmonella in food including immunological enzyme-linked immunosorbent assay (ELISA) based on immunology, polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) based on molecular biology, and matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) based on protein were analyzed and compared. Test methods based on 3 different principles had both advantages and disadvantages. ELISA had low cost, simple operation but high detection limit; PCR had high cost, but rapid detection, high sensitivity and low detection limit; MALDI-TOF MS had low detection limit but need to improve the reservoir. Therefore, fluorescent quantitative PCR with internal amplification control (IAC) and LAMP method as the main methods for rapid detection of Salmonella in food were suggested.