基于肝微粒体和肝S9成分的食品接触材料中 五氯苯酚体外代谢分析

Metabolic study of pentachlorophenol in food contact materials by liver mi-crosome and liver S9 fraction

中文关键词:  五氯苯酚  四氯苯醌  肝微粒体  肝S9成分  气相色谱-质谱联用法  体外代谢

英文关键词:pentachlorophenol  tetrachloro-1,4-benzoquinone  liver microsome  liver S9 fraction  gas chromatography-mass spectrometry  metabolism in vitro

中文摘要:

      目的 通过体外代谢方法, 研究五氯苯酚(pentachlorophenol, PCP)的代谢情况。方法 采用肝微粒体和肝S9成分2种体外代谢试剂模拟体内代谢模式, 通过优化代谢反应条件, 建立PCP、四氯苯醌(Cl4BQ)2种目标物的气相色谱-质谱联用(GC-MS)检测方法, 对PCP进行体外代谢研究分析。结果 体外代谢分析的最佳反应条件为代谢试剂浓度0.75 mg/mL、代谢反应时间4 h。结论 在最优反应条件下分析比较2种试剂对PCP的代谢情况, 验证了PCP的代谢产物为Cl4BQ, 且在肝微粒体和肝S9成分中的代谢没有显著差异。

英文摘要:

      Objective To analyze the metabolite of pentachlorophenol (PCP) through simulating metabolism in vivo. Methods Liver microsomes and liver S9 fraction were used as two kinds of in vitro metabolism reagents to simulate in vivo metabolic pattern. The method for the determination of PCP and tetrachlo-ro-1,4-benzoquinone (Cl4BQ) was established by gas chromatography-mass spectrometry (GC-MS) through optimizing the metabolic reaction conditions, and then in vitro metabolism of PCP was analyzed. Results The best metabolic reagent concentration was 0.75 mg/mL, and the most suitable reaction time was 4 h. Conclusion The two reagents of PCP metabolism under the optimal reaction conditions were compared, Cl4BQ was validated to be the metabolite of PCP, and there was no significant difference between the metabolism composition of liver microsomes and liver S9 fraction.