高效液相色谱-串联质谱法测定兔肉中氟苯尼考 含量的不确定度评估

Assessment of the uncertainty in determination of florfenicol in rabbit meat by high performance liquid chromatography-tandem mass spectrometry

中文关键词:  高效液相色谱-串联质谱法  兔肉产品  氟苯尼考  不确定度

英文关键词:high performance liquid chromatography-tandem mass spectrometry  rabbit meat products  florfenicol  uncertainty

中文摘要:

      目的 评估高效液相色谱-串联质谱法测定兔肉中的氟苯尼考含量的不确定度。方法 依据CNAS-GL06: 2006《化学分析中不确定度的评估指南》和GB/T 20756-2006 《可食动物肌肉、肝脏和水产品中氯霉素、甲砜霉素和氟苯尼考残留量的测定 液相色谱-串联质谱法》, 高效液相色谱-串联质谱法测定兔肉中氟苯尼考含量, 并对不确定度来源进行分析与量化。结果 兔肉中氟苯尼考含量为0.96 μg/kg, 其扩展不确定度为0.23 μg/kg, k=2。结论 兔肉中氟苯尼考含量接近方法的测定低限时, 不确定度对样品的结果有重要参考作用, 因此在结果报告中, 应补充相应的不确定度。

英文摘要:

      Objective To evaluate the uncertainty in determination of the content of florfenicol in rabbit meat by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS). Methods According to the CNAS-GL06: 2006 “Guidance on evaluating the uncertainty in chemical analysis” and GB/T 20756-2006 “Determination of multi-residues of chloramphenicols in the edible animal tissue, liver and Aquatic products”, the main sources of the uncertainty resulted from the determination process of rabbit meat by HPLC-MS were discussed and calculated. Results The content of the florfenicol residue in rabbit meat was 0.96 μg/kg, and the extended uncertainty was 0.23 μg/kg, k=2. Conclusion The content of the florfenicol in rabbit meat is around the detection limit. The uncertainty is very important for the sample result, so in the testing report, it is better to add the uncertainty of measurement.