高效液相色谱法测定食品接触材料中丙烯酸酯类单体的迁移量

Determination of migration amounts of acrylate monomers by high perfor-mance liquid chromatography in food contact materials

中文关键词:  丙烯酸酯类单体  迁移量  食品接触材料  高效液相色谱法

英文关键词:acrylate monomers  migration amount  food contact materials  high performance liquid chromatography

中文摘要:

      目的 建立食品接触材料中12种丙烯酸酯单体迁移量的高效液相色谱检测方法。方法 依据欧盟指令(EU)No. 10/2011, 采用水性模拟物3%乙酸、10%乙醇、20%乙醇、50%乙醇和油性模拟物异辛烷浸泡食品接触材料, 将得到的模拟物通过C18色谱柱(150 mm×4.6 mm, 5 μm)分离, 保留时间定性, 峰面积定量。结果 12种单体在0.3~60.0 mg/L(水性模拟物)及0.6~120.0 mg/L(异辛烷模拟物)的浓度范围内呈良好线性, 相关系数r2大于0.9993。加标回收率在88.2%~109.3%之间, 相对标准偏差为1.19%~7.48%, 方法的检出限和定量限分别在0.01~0.12 mg/L和0.03~0.24 mg/L之间。结论 该方法前处理简便, 分离度好, 分析灵敏度高, 满足食品接触材料中12种丙烯酸酯单体迁移量的分析要求, 并用该方法测定了6批次食品接触材料中丙烯酸酯类单体的迁移水平, 甲基丙烯酸甲酯为主要检出。

英文摘要:

      Objective To develop an effective analytical method for the simultaneous determination of migration amount of 12 kinds of acrylate monomers in food contact materials by high performance liquid chromatography (HPLC). Methods Aqueous food simulants (3% acetic acid, 10% ethanol, 20% ethanol, 50% ethanol) and fatty food simulant (isooctane) were used to soak food contact materials. The obtained simulants were separated on a C18 column(150 mm×4.6 mm, 5 μm). The results were qualitatively analyzed by retention time and quantitatively analyzed by peak area. Results The results showed a good linear correlation in the range of 0.3~60.0 mg/L (aqueous food simulants) and 0.6~120.0 mg/L (isooctane simulant) with correlation coefficients r2 above 0.9993. The recoveries of the ester compounds spiked in the sample and relative standard deviation were 88.2%~109.3% and 1.19%~7.48% respectively. The limit of detection and limit of quantity of the method were between 0.01~0.12 mg/L and 0.03~0.24 mg/L respectively. Conclusion The method is simple with good separation and high analysis sensitivity, and meets the analysis requirements of 12 kinds of acrylate monomers migration in food contact materials. The method has been applied to determine the migration levels of acrylate monomers in different simulants from the migration tests of 6 batches of food contact material samples, and methyl methacrylate was found in the majority of the samples.