放射免疫法快速检测牛血清中四环素类药物

Detection of tetracycline in bovine serum with radioimmunoassay method

中文关键词:  放射免疫分析方法  四环素类  牛血清

英文关键词:radioimmunoassay method  tetracycline  bovine serum

中文摘要:

      目的 采用Charm II 放射免疫分析方法检测牛血清中四环素类药物残留。方法 采集6个牛血清样品进行添加检测, 设定控制点, 样品结果与控制点比较。评价免疫反应体系的特异性和灵敏度。测得控制点后与样品结果比较, 大于控制点则为阴性, 小于控制点则为阳性。结果 采用此方法短时间可出检测结果, 四环素类检测限为20 μg/kg。该方法特异性强, 操作方法简单便捷。结论 此方法作为初筛方法, 测得阳性结果时则需采用其他检测方式进一步确证。该方法保持了家畜鲜活状态, 减少了商家的损失, 既能快速得到检测结果, 又能达到检测牛血清中四环素类药物残留的要求。

英文摘要:

      Objective To detect the sulfonamide residues in bovine serum by Charm II radioimmunoassay method. Methods Six serum samples were collected for the addition test. The control point was set. The results from the sample test were compared with the control point. The sensitivity and specificity of the immune reaction system was evaluated. The measured value was compared with the control point. The sample was de-termined to be negative if the measured value was greater than the control point, whereas, the sample was positive if the measured value was less than the control point. Results Test results can be obtained in short time and the limit of tetracycline detection was 20 μg/kg. The method was highly specific, easy and convenient to operate. Conclusion Other test methods are still needed to further confirm the positive results as the method proposed in this paper is only a preliminary method. This method can keep livestock alive and reduce losses of merchants. It can quickly give out test results and meet detection requirements of tetracycline residues in bovine serum.