榆干离褶伞溶栓酶对内皮细胞损伤大鼠模型的保护作用

Protective effects of fibrinolytic enzyme from Lyophyllum ulmarium on vascular endothelial cells injury in rats

中文关键词:  榆干离褶伞  溶栓酶  血管内皮细胞

英文关键词:Lyophyllum ulmarium  fibrinolytic enzyme  vascular endothelium

中文摘要:

      目的：探讨榆干离褶伞溶栓酶对肾上腺素致血管内皮细胞损伤的保护作用。方法：采用注射肾上腺素结合冰浴的方法制备大鼠血管内皮细胞损伤模型，检测血液循环内皮细胞数（CEC）和血浆血栓素B2 (TXB2)、6-酮-前列腺素E1α(6-keto-PGE1α)、组织纤溶酶原激活物（t-PA）以及抗凝酶Ⅲ(AT-Ⅲ)的含量。结果：模型组的循环内皮细胞数和TXB2水平较正常组明显增高，且t-PA和AT-Ⅲ含量下降。用药组可减少循环内皮细胞数和TXB2水平，提高6-keto-PGE1α、t-PA和AT-Ⅲ含量。结论：榆干离褶伞溶栓酶对血管内皮细胞起保护作用。

英文摘要:

      Objective: To study the protective effect of fibrinolytic enzyme from Lyophyllum ulmarium (L.u) on endothelial cell injury induced by adrenaline. Methods: Rats were injected with adrenaline and making swim in ice-cold water. Then the number of circulating endothelial cells, and the levels of TXB2, 6-keto-PGE1α, t-PA and AT-Ⅲ in plasma were measured. Results: Model group increased the number of circulating endothelial cells and TXB2, and decreased the levels of t-PA and AT-Ⅲ as compared with the normal group. The treatment group reduced the number of circulating endothelial cells and TXB2 content, increase the levels of t-PA and AT-Ⅲ as compared with the model group. Conclusion: The fibrinolytic enzyme from Lyophyllum ulmarium presents the protective effect on vascular endothelial cells.