伏马毒素B1新型荧光标记物制备及其在荧光偏振分析技术中的应用

Preparation and application of new fluorescein-labeled fumonisins B1 in flu-orescence polarization analysis technique

中文关键词:  伏马毒素B1  氨基荧光素  荧光标记物  荧光偏振

英文关键词:fumonisins B1  aminofluorescein  fluorescent tracer  fluorescence polarization

中文摘要:

      目的 针对玉米中常见真菌毒素——伏马毒素B1(FB1)开发新型荧光标记物, 从而替代昂贵的6-(4,6-二氯三嗪基)氨基荧光素(6-DTAF)标记物, 并在此基础上开发基于荧光偏振原理的伏马毒素快速检测技术。方法 采用一锅煮的合成方法, 以5-(4,6-二氯三嗪基)氨基荧光素(5-DTAF)为荧光基团制备伏马毒素荧光标记物, 然后利用荧光偏振的原理建立基于5-DTAF的伏马毒素快速检测技术。结果 基于5-DTAF的FB1荧光标记物与基于6-DTAF的FB1荧光标记物的荧光偏振分析方法具有相同的分析性能, IC50值基本相等。结论 采用5-DTAF成功制备了FB1荧光标记物, 同时利用该荧光标记物开发了伏马毒素荧光偏振快速检测技术。该分析技术与基于6-DTAF荧光标记物的FB1荧光偏振速测技术相比, 具有同样的分析性能, 但分析成本降低了一半。

英文摘要:

      Objective To Prepare a new fluorescent tracer against common mycotoxins such as fumonisins B1 for replace 6-(4,6-Dichlorotriazinyl) aminofluorescein (6-DTAF) expensive marker, and to develop a technique for quick detection of fumonisins B1 based on the principle of fluorescence polarization. Methods The one-pot method was used to synthesize 5-(4,6-Dichlorotriazinyl) aminofluorescein (5-DTAF) fluorescent marker, and then fluorescence polarization technique based on 5-DTAF was established to detect fumonisins B1. Results Fluorescence polarization technology based on 5-DTAF and 6-DTAF fluorescent marker of fumonisins B1 had almost the same analysis capability and the value of IC50. Conclusion New fluorescent marker of fumonisins B1 was successfully synthesized by using 5-DTAF, and fluorescence polarization technology for fumonisins B1 quick detection was developed. Moreover, the fluorescence polarization technology based on 5-DTAF fluorescent marker had almost the same analysis capability comparing with that of 6-DTAF fluorescent marker, but cut the analysis cost to a half.