QuEChERS-超高效液相色谱-串联质谱法快速测定黄豆芽中的6-苄基腺嘌呤

Determination of 6-benzylaminopurine in soybean sprouts by QuEChERS-UHPLC-MS/MS method

中文关键词:  QuEChERS  超高效液相色谱-串联四极杆质谱法  黄豆芽  6-苄基腺嘌

英文关键词:QuEChERS  high performance liquid chromatography-tandem mass spectrometry  soybean sprouts  6-benzylaminopurine

中文摘要:

      目的 建立黄豆芽中的6-苄基腺嘌呤的超高效液相色谱-串联四极杆质谱测定方法。方法 黄豆芽样品经乙腈超声提取后, 经C18和PSA的固相分散净化后进样分析。使用SB-Aq色谱柱分离, 以水-乙腈为流动相进行梯度洗脱。采用电喷雾离子化源, 以多反应监测(multiple reaction monitoring)方式分别在负离子模式下进行检测。结果 6-苄基腺嘌呤的线性范围为5~200 μg /kg, 相关系数良好, 检出限为0.5 μg/kg, 该方法在三个水平上添加回收率在88.1%~107%, 相对标准偏差在1.4%~13.4%。结论 本方法灵敏度高, 操作简单高效, 适合于黄豆芽中的6-苄基腺嘌呤的定量及确证分析。

英文摘要:

      Objective To establish an ultra-high pressure liquid chromatography (UHPLC) coupled to tandem mass spectrometry (MS/MS)method for the determination of 6-benzylaminopurine in soybean sprouts. Methods The analyte was extracted from homogenized soybean sprouts samples with acetonitrile, and solid phase dispersed by C18 and PSA. The chromatography separation was performed on a SB-Aq column by ace-tonitrile and water as mobile phase in gradient elution program．Then samples were determined by electrospray ionization in negative mode using multiple reactions monitoring(MRM). Results A good linear relationship was obtained in range of 5~200 μg /kg, the limit of detection was 0.5 μg/kg. The recoveries at 3 spiked levels were between 88.1%~107%, with the relative standard deviations (RSDs) between 1.4%~13.4% (n=6). Conclusion The method was sensitive, effective, and simple, and was suitable for the determination and confirmation of 6-benzylaminopurine in soybean sprouts.