长牡蛎中类HBGAs的分型及与诺如病毒P粒子 结合特性研究

Type-like of HBGAs in Crassostrea gigas and its binding properties with norovirus P particles

中文关键词:  长牡蛎  组织血型抗原  诺如病毒  结合特性

英文关键词:Crassostrea gigas  histo-blood group antigens  norovirus  binding properties

中文摘要:

      目的 了解长牡蛎(Crassostrea gigas)消化道组织中类组织血型抗原(histo-blood group antigens, HBGAs)的类型特点, 分析其与诺如病毒的结合特性, 以探讨长牡蛎富集诺如病毒的机制。方法 利用8种HBGAs单克隆抗体, 建立长牡蛎中类HBGAs检测的ELISA方法, 并分析其主要型别。同时, 利用5种体外表达的GII.4型诺如病毒P粒子分析其与长牡蛎中类HBGA的结合特性。 结果 长牡蛎消化道组织中存在类A, H1, Lea和Ley型HBGA; 55019株(2006b变异株)和97-1l株(95/96US变异株) GII.4型诺如病毒P粒子可通过类A、H1和Ley型HBGA与长牡蛎消化道组织相结合, 91(Camberwell_91株)和42(Hunter_2004)株可通过类Ley型HBGA与长牡蛎消化道组织相结合, 156株(sakai株)不与任何类型类HBGA结合。结论 长牡蛎消化道组织中存在类A、H1、Lea和Ley型HBGA, GII.4型诺如病毒主要通过类A、H1和Ley型HBGA与长牡蛎消化道组织相结合。

英文摘要:

      Objective To know the features of type-like HBGAs in digestive tissue of Crassostrea gigas, and analyze its binding properties of GII.4 genotype norovirus variants, and to research the mechanism of enrichment. Methods Eight types HBGAs-specific monoclonal antibodies (MAbs) from human were used to establish the ELISA method for type-like HBGAs in Crassostrea gigas. Binding profile between type-like HBGAs in Crassostrea gigas and 5 GII.4 genotype norovirus variants were also analyzed. Results A-like, H1-like, Lea-like and Ley-like were the main types HBGAs existed in digestive tissue of Crassostrea gigas. 2006b variant and 95/96US variant binding with A-like, H1-like and ley-like HBGAs in Crassostrea gigas. However, Camberwell_91 variant and Hunter_2004 variant only bind with ley-like HBGA, and sakai variant bind no type HBGA in Crassostrea gigas. Conclusion There are A-like, H1-like, Lea-like and Ley-like type HBGAs in digestive tissue of Crassostrea gigas. GII.4 genotype norovirus variants mainly bind with A-like , H1-like and Ley-like HBGA in Crassostrea gigas.