连续光源原子吸收光谱法顺序测定蛤蚧中 6种金属元素

Sequential determination of Cu, Fe, Zn, Mn, Ni and Cr in different parts of Gekko gecko L. by continuum source atomic absorption spectrometry

中文关键词:  蛤蚧  连续光源  原子吸收光谱法  金属元素  顺序测定

英文关键词:Gekko gecko L.  continuum source  atomic absorption spectrometry  metallic element  sequential determination

中文摘要:

      目的 采用连续光源原子吸收光谱法顺序测定蛤蚧头、躯干、尾、四肢等不同部位中铜、铁、锌、锰、镍、铬等6种金属元素的含量。方法 采用微波消解样品, 连续光源原子吸收光谱技术多元素顺序测定的方法。结果 优化条件下, 在0.05~2.00 mg/L浓度范围内, 各元素的吸光度与浓度呈良好的线性关系(相关系数r≥0.9993), 本方法检出限在0.3~13.2 μg/g之间, 加标回收率在76.7%~100.0%之间。将该法应用于实际蛤蚧样品中上述元素的测定, 测定结果表明, 蛤蚧不同身体部位中6种元素含量有所不同, Fe、Mn元素在四肢中的含量最高, Cu、Zn、Cr元素在头部中含量最高, Ni元素在躯干中的含量最高。结论 本方法满足实际样品的分析要求, 且较之常规锐线光源原子吸收光谱技术在保证分析准确度的同时, 大大提升了分析效率。本方法可以对中药重金属污染风险监测提供借鉴。

英文摘要:

      Objective To determine Cu, Fe, Zn, Mn, Ni and Cr content sequentially in different parts of Gekko gecko L. by continuum source atomic absorption spectrometry (CS-AAS). Methods CS-AAS technique assisted with microwave digestion were adapted to determine 6 kinds of metallic element. Results Under the optimized conditions, the absorbance of each element was in favorable linear relationship with the con-centration in the range of 0.05~2.00 mg/L, the correlation coefficients were no less than 0.9993. The detecting limits for all the element of the proposed method were in the range of 0.3~13.2 μg/g. The spiked recoveries for the Gekko samples were in the range of 76.7%~100.0%. This method was also used to analyze real samples, and it showed that the content of 6 kinds of element in different parts of Gekko were diverse. The content of Fe and Mn were higher in limbs, the content of Cu, Zn and Cr were higher in head, and the content of Ni was higher in body. Conclusion Compared with traditional sharp line source AAS technique, the analytic efficiency was obviously improved by this method. Reference can be provided by this method for heavy metal pollution risk monitoring of traditional Chinese medicine.