高效液相色谱法测定油炸蚕豆中的多菌灵残留

Determination of carbendazim residue in fried broad bean by high performance liquid chromatography

中文关键词:  高效液相色谱法  油炸蚕豆  多菌灵  固相萃取

英文关键词:high performance liquid chromatography  fried broad bean  carbendazim  solid-phase extraction

中文摘要:

      目的 建立高效液相色谱法测定油炸蚕豆中的多菌灵残留的方法。方法 试样经1%乙酸-乙腈溶液提取, MCX固相萃取柱净化, 甲醇溶解定容, 经C18色谱柱分离, 以乙腈-水为流动相, 在波长274 nm处检测, 外标法定量。结果 在质量浓度为0.10～4.0 mg/L范围内, 峰面积与其浓度的线性关系良好, 相关系数为0.9998, 方法检出限为0.02 mg/kg, 平均回收率为85.2%～95.9%。结论 该方法简便、快速准确, 适用于油炸蚕豆中多菌灵的测定。

英文摘要:

      Objective To study and establish a method for determination of carbendazim residue in fried broad bean by high performance liquid chromatography. Methods The sample was extracted by 1% acetic acid-acetonitrile, cleaned up by MCX SPE, dissolved and volumed by methanol, and then separated by C18 chromatography column, the mobile phase was acetonitrile-water, the detection wavelength was 274 nm, and the quantitave method was the external standard method. Results The linear relationship between peak area and concentration was good over the range of 0.10~4.0 mg/L with the correlation coefficient 0.9998, and the detection limit was 0.020 mg/kg, the average recoveries of the method were ranged from 85.2%~95.9%. Conclusion This method is easier, faster and suitable for the determination of carbendazim in fried broad bean.