高效液相色谱-蒸发光散射检测法快速测定益生菌粉中低聚果糖含量

Rapid determination of fruto-oligosaccharides content in probiotic powder by high performance liquid chromatography-evaporative light-scattering detector

中文关键词:  低聚果糖  高效液相色谱-蒸发激光散射检测法  益生菌粉

英文关键词:fruto-oligosaccharides  high performance liquid chromatography-evaporative light-scattering detector  probiotic powder

中文摘要:

      目的 建立高效液相色谱-蒸发光散射检测(HPLC-ELSD)法, 快速洗脱分离出蔗果三糖、蔗果四糖、蔗果五糖, 测定益生菌粉中低聚果糖的含量。方法 样品经过前处理, 以乙腈和水为流动相, 等度洗脱, 经XAmide (4.6 mm×250 mm,5 μm) 中性酰胺色谱柱分离, 采用蒸发激光检测器检测。结果 蔗果三糖、蔗果四糖、蔗果五糖线性范围分别在0.2671～3.168 mg/mL、0.2429～2.8215 mg/mL、0.1617～3.544 mg/mL, 线性相关系数r＞0.99。3种主要组成的回收率在97.0%～105.0%之间。结论 该方法可有效分离出蔗果三糖、蔗果四糖、蔗果五糖, 推广性高、快速、简便、准确, 可用于益生菌粉中低聚果糖含量的测定。

英文摘要:

      Objective To establish a rapid determination for the rapid separate kestose, nystose and 1F-fructofuranosyl nystose by high performance liquid chromatography-evaporative light-scattering detector (HPLC-ELSD) method, and the concentration of fruto-oligosaccharides can be determined in probiotics powder. Methods After pretreatment of samples, acetonitrile and water as the mobile phase, while the elution method was gradient elution, the samples were separated by XAmide (4.6 mm×250 mm,5 μm) neutral amide chromatographic column, and detected by evaporative laser detector. Results The linear ranges of kestose, nystose and 1F-fructofuranosyl nystose were from 0.2671 to 3.168 mg/mL, 0.2429 to 2.8215 mg/mL and 0.1617 to 3.544 mg/mL, respectively, and the linear correlation coefficient r＞0.99. The recovery rate of the 3 compositions was between 97.0%～105.0%. Conclusion This method can effectively separate the kestose, nystose and 1F-fructofuranosyl nystose. It is a high generalization, rapid, simple and accurate method, and can be applied for the determination of fruto-oligosaccharides in probiotics powder.