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曾静,刘莉,周雪婷,周熙城,张琳,张西萌,马丹,魏海燕,倪元颖.单核细胞增生李斯特氏菌食品分离株协同溶血现象研究[J].食品安全质量检测学报,2015,6(11):4355-4357

单核细胞增生李斯特氏菌食品分离株协同溶血现象研究

Christie, atkins and munch-petersen for Listeria monocytogenes isolated from food

投稿时间：2015-05-25 修订日期：2015-11-12

DOI：

英文关键词:Listeria monocytogenes christie, atkins and munch-petersen Staphylococcus aureus Rhodococcus equi

基金项目:国家质检总局公益性行业科研专项(201110034)

作者	单位
曾静	北京出入境检验检疫局
刘莉	北京出入境检验检疫局
周雪婷	北京出入境检验检疫局;中国农业大学食品科学与营养工程学院
周熙城	北京出入境检验检疫局
张琳	河北出入境检验检疫局
张西萌	北京出入境检验检疫局
马丹	北京出入境检验检疫局
魏海燕	北京出入境检验检疫局
倪元颖	中国农业大学食品科学与营养工程学院

Author	Institution
ZENG Jing	Beijing Entry-Exit Inspection and Quarantine Bureau
LIU Li	Beijing Entry-Exit Inspection and Quarantine Bureau,
ZHOU Xue-Ting	Beijing Entry-Exit Inspection and Quarantine Bureau; College of Food Science & Nutritional Engineering, China Agricultural University
ZHOU Xi-Cheng	Beijing Entry-Exit Inspection and Quarantine Bureau,
ZHANG Lin	Hebei Entry-Exit Inspection and Quarantine Bureau
ZHANG Xi-Meng	Beijing Entry-Exit Inspection and Quarantine Bureau,
MA Dan	Beijing Entry-Exit Inspection and Quarantine Bureau,
WEI Hai-Yan	Beijing Entry-Exit Inspection and Quarantine Bureau,
NI Yuan-ying	College of Food Science & Nutritional Engineering, China Agricultural University

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中文摘要:

目的对从食品中分离的88株单核细胞增生李斯特氏菌(Listeria monocytogenes)进行协同溶血(christie, atkins and munch-petersen, cAMP)测试。方法根据食品安全国家标准食品微生物学检验单核细胞增生李斯特氏菌检验GB 4789.30-2010的方法进行cAMP测试。结果所测试的88株单核细胞增生李斯特氏菌分离株与GB 4789.30中描述的结果一致, 即在靠近金黄色葡萄球菌(Staphylococcus aureus)的接种端溶血增强, 78株靠近马红球菌一端呈阴性反应。同时, 发现10株分离株cAMP测试结果与传统的测试结果不同, 在靠近马红球菌(Rhodococcus equi)的接种端溶血增强。结论对88株单核细胞增生李斯特氏菌分离株cAMP测试结果表明, 有大约11%的菌株在靠近马红球菌的接种端溶血增强。

英文摘要:

Objective To do the christie, atkins and munch-petersen (cAMP) testing for Listeria monocytogenes isolates from food. Methods The GB 4789.30-2010 method was used for cAMP test. Results Cha-racterization on all 88 food isolates by cAMP test revealed that 88 strains showed characteristics enhance-ment of haemolytic zone with Staphylococcus. aureus, meanwhile 78 strains showed negative reaction with Rhodococcus. equi and another 10 strains showed characteristics enhancement of haemolytic zone with R. equi. Conclusion In this study, there are about 11% L. monocytogenes isolated from food showing characteristics enhancement of haemolytic zone with R. equi.

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