能力验证菌落总数测定结果不确定度的评定

Uncertainty evaluation of aerobic plate count by proficiency testing

中文关键词:  能力验证  菌落总数  不确定度评定

英文关键词:proficiency testing  aerobic plate count  uncertainty evaluation

中文摘要:

      目的 对实验室能力验证样品菌落总数进行不确定度评定。方法 能力验证菌落总数依据GB 4789.2-2010《食品安全国家标准食品微生物学检验 菌落总数测定》以及能力验证计划参试指导书来进行测定和结果判断, 再根据JJF 1059.1-2012《测量不确定度评定与表示》的规定, 对测定过程中引入的不确定度分量进行评定, 然后采用合成的方法来计算和评定菌落总数的不确定度。结果 菌落总数测定结果合成不确定度为0.05042, 扩展不确定度为0.1008(以对数计)。结论 本研究建立的方法可以对能力验证样品的菌落总数进行不确定度评定, 此方法适合于类似检测条件下菌落总数不确定度的评定。

英文摘要:

      Objective To evaluate the uncertainty of aerobic plate count by proficiency testing. Methods Proficiency testing of aerobic plate count was conducted under the direction of GB 4789.2-2010 National food safety standard microbiological examination in food: Aerobic plate count and CFAPA-024 Reference book for microbiological ability verification plan in food. Then, the uncertainty components introduced in the experiment were determined according to JJF1059.1-2012 Evaluation and expression of uncertainty in measurements. Finally, synthetic method was used to calculate and assess the uncertainty of aerobic plate count. Results The combined uncertainty of aerobic plate count was 0.05042, the expanded uncertainty was U(1og)=0.1008. Conclusion The method established in this study can be used in the uncertainty evaluation of aerobic plate count under similar circumstances.