液相色谱-串联质谱法检测豆制品中的二乙基黄

Determination of diethyl yellow in bean products by liquid chromatogra-phy-tandem mass spectrometry

中文关键词:  液相色谱-串联质谱法  二乙基黄  豆制品

英文关键词:liquid chromatography-tandem mass spectrometry  diethyl yellow  bean products

中文摘要:

      目的 建立一种简单、快速的豆制品中二乙基黄的液相色谱-串联质谱检测方法。方法 样品经乙腈超声辅助提取和离心分离, 进样后目标物经由C18柱分离, 0.1%甲酸水溶液和乙腈做流动相梯度洗脱, 采用电喷雾离子源正离子模式下选择离子反应监测(SRM)。结果 本法对二乙基黄的检出限为0.5 μg/kg, 加标回收率为85.5%~103.2%, 相对标准偏差(RSD)为1.3%~4.9%(n=6), 且二乙基黄在0.05~10 ng/mL浓度范围内呈良好的线性关系, 线性回归系数(r)可达0.9997。结论 本法适用于对豆制品中二乙基黄的快速检测。

英文摘要:

      Objective To establish a simple and rapid method for determination of diethyl yellow in bean products by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods Samples were extracted with acetonitrile using ultrasonic-assisted extraction and separated by centrifugation. After injection, the chromatographic separation was achieved on a C18 column using a mobile phase composed of a mixture of 0.1% formic acid and acetonitrile through gradient elution, and determined by electrospray mass spectrometry in the positive mode in selected ion monitoring mode (SRM). Results The limit of detection of diethyl yellow were 0.5 μg/kg. The recoveries for them spiked at three levels respectively ranged from 85.5 % to 103.2 %, and the RSDs were 1.3%~4.9% (n=6). The linear ranges of this method were 0.05~10.0 ng/mL and the correlation coefficients (r) were 0.9997. Conclusion The method was suitable for determination of diethyl yellow in bean products .