高效液相色谱法测定食品中苏丹红I~IV

Determination of Sudan I~IV in food by high performance liquid chroma-tography

中文关键词:  高效液相色谱法  苏丹红  食品

英文关键词:high performance liquid chromatography  Sudan dyes  food

中文摘要:

      目的 建立准确可靠的高效液相色谱法测定食品中苏丹红I~IV的方法。方法 用乙腈提取样品中的苏丹红, 经漩涡超声离心后, 将乙腈层倾倒出, 用乙腈重复提取, 通过凝胶色谱的净化, 用液相色谱进行检测。其中凝胶色谱柱为Bio-BeadsTM S-X3 Beads, 液相色谱柱为Waters C18 (4.6 mm×150 mm, 5 μm), 液相色谱流动相为乙腈-水(V:V, 90:10)。结果 4种苏丹红染料的检出限均能达到10 μg/kg, 满足国标要求。标准加入量为0.16 μg/mL浓度时, 辣椒粉的回收率为92.5%~97.5%、辣椒酱的回收率为83.8%~87.5%、香肠的回收率为82.5%~98.8%, 相关系数0.9995以上, 且重复性良好。结论 高效液相色谱法可以测定食品中苏丹红I~IV, 适用于批量样品的检测。

英文摘要:

      Objective To establish an accurate and reliable determination method of Sudan I, II, III, and IV in food by HPLC. Methods The Sudan in samples were extracted with acetonitrile, after through ultrasonic, vortex and centrifugation, and the layer was poured and repeated processing with acetonitrile, and purified by GPC. The Sudan were detected by liquid chromatography. In this study, GPC column was Bio-BeadsTM S-X3 Beads, and liquid chromatographic column was Waters C18 (4.6 mm×150 mm, 5 μm), acetonitrile-water as the mobile phase was used in isocratic conditions (V:V, 90:10). Results The limit of detection (LOD) of method was 10 μg/kg, which met the requirements of the national standard. Recoveries of Sudan dyes in chilli powder ranged from 92.5% to 97.5%, chili sauce ranged from 83.8% to 87.5% and sausage ranged from 82.5% to 98.8% with a good reproducibility at a spiking level of 0.16 μg/mL. Conclusion This method was applicable for batch sample to determine Sudan I~IV.