液质联用法对鱼类水产品中孔雀石绿的调查分析

Investigation of malachite green in aquatic fish by liquid chromatography- mass spectrometry/mass spectrometry

中文关键词:  孔雀石绿  隐色孔雀石绿  高效液相色谱-串联质谱法

英文关键词:malachite green  leucomalachite green  liquid chromatography mass spectrometry-mass spectrometry

中文摘要:

      目的 通过对本辖区学校食堂和水产市场的鱼类水产品进行孔雀石绿的监测, 调查孔雀石绿在不同品种鱼类体内的污染状况。方法 选用Agilent ZORBAX Eclipse Plus C18色谱柱, 以10 mmol/L甲酸铵-0.1%甲酸:乙腈为流动相, 采用梯度洗脱进行分离。鱼肉样品中加入内标和酸性氧化铝, 用乙腈提取, 多重态反应监测(MRM)方式检测。结果 238份涉及共9个品鱼肉样本中, 阳性率为16.2%~71.4%, 其中桂花鱼、鲩鱼、鲫鱼三种阳性率相对较高。结论 本方法样品处理简便、检测结果准确可靠, 孔雀石绿在淡水鱼的养殖和运输中仍有滥用, 其含量与环境温度、鱼的品种有一定关系。

英文摘要:

      Objective To investigate the malachite green pollution in different varieties of fish by monitoring of malachite green in fish aquatic products in the area of school canteens and aquatic products market. Methods Malachite green was extracted from fish muscle by acetonitrile with adding internal standard compound and acidic alumina, and then separated by Agilent ZORBAX Eclipse Plus C18 column with 10 mmol/L ammonium formate-0.1% formic acid and acetonitrile as mobile phase by gradient elution. Detection was carried out by multiple reaction monitoring. Results In 238 specimens involving 9 kind of fish, the positive rate was from 16.2% to 71.4%, among which the positive rate of mandarin fish, grass carp and crucian were relatively higher. Conclusion The method is accurate and reliable with simple sample preparation. Malachite green is still abused in freshwater fish breeding and transportation, and its content has certain relationship between the environment temperature and the varieties of fish.