西地那非多克隆抗体的制备及特异性分析

Preparation and specificity analysis of sildenafil polyclonal antibody

中文关键词:  西地那非  多克隆抗体  酶联免疫

英文关键词:sildenafil  polyclonal antibody  ELISA

中文摘要:

      目的 为了研究开发一种具有高灵敏度、高特异性的西地那非(sildenafil, SD)多克隆抗体。方法 本文通过人工合成西地那非半抗原(SDH)和西地那非抗原(SDH-BSA), 然后经免疫兔子, 制备了SD多克隆抗体, 并对制得的抗体的特异性进行了分析。结果 免疫后产生了SD多克隆抗体, 抗体的效价为3.8×105, 线性检测范围为0.02~3.8 ng/mL, 抗体除与瓦地那非具有较高的交叉反应率外, 与红地那非、他地那非等结构类似物交叉反应率均较低。结论 采用本方法制备的SD多克隆抗体具有较高的特异性和灵敏度, 可用于SD快速检测试剂盒的开发。

英文摘要:

      Objective To prepare high sensitivity and specificity polyclonal antibody against sildenafil (SD). Methods Sildenafil hapten (SDH) and Sildenafil antigen (SDH-BSA) were prepared by artificial methods. Then, through immunized rabbits the SD polyclonal antibody was prepared. Results After immunity, the polyclonal antibody against SD was generated. The titer of the antibody was 3.8×105. The linear detection of the polyclonal antibody was ranged from 0.02 ng/mL to 3.8 ng/mL. The polyclonal antibody had a high cross reaction rate to vardenafil, but had a low response rate to hongdenafil and calais. Conclusion SD polyclonal antibody had a high specificity and sensitivity, and it could be applied to the development of SD rapid detection kit.