直接落射荧光/平板计数法检测辐照中草药

Identification of irradiation of herbal medicine by direct epifluorescent filter technique-aerobic plate count method

中文关键词:  辐照  中草药  直接荧光过滤  平板计数

英文关键词:irradiation  herbal medicine  direct epifluorescent filter technique  aerobic plate count method

中文摘要:

      目的 建立一种适用于中草药辐照检测方法。方法 对白蔻、紫苏、忍冬藤、甘草与荷叶5种中草药样品分别在4 kGy、5 kGy及10 kGy剂量下进行辐照处理, 同时以未辐照样品作为对照。辐照后分别测试每份样品的直接落射荧光计数单位(DEFT值)和细菌总数(APC值), 并计算DEFT值与APC值之差(Dc值), 将该差值与特定阈值比较判定样品辐照情况。结果 随辐照剂量增加, DEFT值差异不显著, DEFT值分别为0 kGy: 6.9~9.0, 4 kGy: 6.8~8.7, 5 kGy: 6.7~8.9, 10 kGy: 6.9~9.0; APC值逐渐降低, APC值分别为0 kGy: 6.0~7.8, 4 kGy: 3.8~5.6, 5 kGy: 3.2~5.5, 10 kGy: 1.2~3.7; 辐照后4组样品之间Dc值差异显著, 4 kGy、5 kGy、10 kGy及0 kGy下辐照处理样品的Dc均值分别为2.6~4.9, 3.0~5.3, 5.3~7.3和0.4~2.0。结论 当阈值设定为3.0时, 可检测出5.0 kGy及5.0 kGy以上辐照处理样品。

英文摘要:

      Objective To establish a screening method for detecting irradiation of herbal medicine in China. Methods Samples of 5 kinds of herbal medicine, including cardamon, purple perilla, honeysuckle stem, liquorice, and lotus leaf, were irradiated with the doses of 4 kGy, 5 kGy and 10 kGy, one group of unirradiated samples were kept as the 0 kGy. The direct epifluorescent filter technique count unit (DEFT value), aerobic plate count unit (APC value), the difference of DEFT and APC (DC value) were calculated after irradiation. Then the DC value was compared to a special threshold to determine the irradiation situation of the samples. Results The results indicated that with the irradiation dose increased, the difference of DEFT was not significant, the DEFT value was 0 kGy: 6.9~9.0, 4 kGy: 6.8~8.7, 5 kGy: 6.7~8.9, 10 kGy: 6.9~9.0; and the APC value was decreased, the APC value was 0 kGy: 6.0~7.8, 4 kGy: 3.8~5.6, 5 kGy: 3.2~5.5, 10 kGy: 1.2~3.7; the Dc values of the 4 groups were statistically significant, the Dc values of 4 kGy, 5 kGy, 10 kGy and 0 kGy was 2.6~4.9, 3.0~5.3, 5.3~7.3 and 0.4~2.0 respectively. Conclusion When 3.0 was set as the threshold, the samples irradiated with dose of 5 kGy and up could be identified.