多重基因遗传表达分析系统及其应用研究进展

Development of gene expression profiler genetic analysis system and its application

中文关键词:  多重基因表达分析系统  多重PCR  微生物检测  食品检测

英文关键词:gene expression profiler genetic analysis system  multiplex-PCR  detection of foodstuffs  detection of microbe

中文摘要:

      核酸检测技术广泛用于生命科学、微生物学及医学领域。随着分子生物学的发展, 在经典技术的基础上研究人员又开发出多种新型检测技术。其中多重基因遗传表达分析系统(gene expression profiler genetic analysis system, GeXP)技术作为聚合酶链式反应和芯片的换代技术, 具有特异性好、灵敏度高的特点, 能够同时检测多个目的基因, 真正意义上实现了高通量检测。本文详细介绍了GeXP技术的原理以及优缺点, 并对GeXP技术在畜牧兽医研究、转基因食品检测、微生物检测及医学研究中的应用进行阐述, 以期为该技术将来在食品检测方面的推广提供参考。

英文摘要:

      Detection of nucleic acid is widely used in life science, microbiology and medical science. With the developments of molecular biology, new testing approaches are constantly emerging on the basis of classic-al technology. As the regeneration technology of PCR and chip, gene expression profiler genetic analysis sys-tem (GeXP) has a broad application prospect, with features of high specificity and sensitivity, rapid speed and easy operation. This system truly realized the detection of high throughput for samples. This article reviewed the basic principle of GeXP system and its applications in veterinarian and animal husbandry, detection of genetically modified (GM) food and microorganism and medical research, which could provide a reference for detection of foodstuffs and microbiology.