离子色谱法测定香肠中亚硝酸盐含量的不确定度评定

Evaluation of uncertainty in ion chromatography determination of nitrite in sausage

中文关键词:  不确定度  离子色谱法  香肠  亚硝酸盐

英文关键词:uncertainty  ion chromatography  sausage  nitrites

中文摘要:

      目的 建立离子色谱法测定香肠中亚硝酸盐的不确定度评定方法, 给出不确定度, 为简化评定方法和提高检测质量提供依据。方法 应用测量不确定度理论, 建立检测方法的数学模型, 找出影响不确定度的因素并对各个不确定度分量进行评估。结果 由各不确定度分量评定结果可以看出香肠中亚硝酸盐的相对扩展不确定度, 校准曲线的不确定度分量较大, 合成的扩展不确定度为0.26 mg/kg。结论 提高校准曲线制作精度是离子色谱法检测香肠中亚硝酸盐的重要质控点, 在检测中可以通过设计合理的校准曲线和增加校准曲线的测量次数来减小不确定度带来的影响。通过对香肠中亚硝酸盐含量的测量不确定度的评定, 对检测结果可以给出一个正负区间, 提供一个准确的检测结果, 提高了检测结果的可信度; 同时建立了离子色谱定量分析不确定度的通用模式, 对检测实验室的不确定度评估起到一定的指导意义。

英文摘要:

      Objective To establish ion chromatography(IC) uncertainty assessment method for determining the concentration of nitrite in sausage and provide the basis for simplification of assessment method and evaluation of detecting results. Methods The calculation formula of the uncertainty was deduced, and factors of uncertainty was analyzed and calculated with uncertainty theory. Results Nitrite in sausage relative expanded uncertainty by each component of uncertainty evaluation results could be seen, the uncertainty of calibration curve components was larger, Synthesis of expanded uncertainty was 0.26 mg/kg. Conclusion To improve the manufacturing accuracy was an important quality control point about detection of nitrite in sausage by ion chromatography calibration curve, the effects of uncertainty was reduced by reasonable design of calibration curve and increased the number of calibration curve. Through measuring the content of nitrite in sausage evaluation of uncertainty, the testing results could be given a negative region, and provide an accurate detection results, and improve the reliability of detection; at the same time,a general model of the uncertainty of the quantitative analysis by ion chromatography was established, it had a certain guiding significance in the testing laboratory evaluation of uncertainty.