气质联用法快速检测中毒患者体液中毒鼠强

Determination of the body fluids from tetramine poisoning patients by gas chromatography-mass spectrometry

中文关键词:  气质联用  快速检测确证  胃内容物  血液  尿液  毒鼠强中毒

英文关键词:gas chromatography-mass spectrometry  rapid detection confirmation  gastric content  blood  urine  tetramine poisoning

中文摘要:

      目的 建立毒鼠强中毒患者的胃内容物、血液和尿液中快速检测毒鼠强的气质联用方法。方法 运用C18小柱对胃内容物与血液进行除杂、净化后, 通过气质联用仪上的大体积进样与程序升温进样, 并利用小口径毛细管色谱柱HP-5MS(0.18 mm×20 m, 0.18 μm)进行检测, 用选择离子模式对m/z 92、132、212、240进行采集。结果 从样品前处理到确证完成全过程耗时约20 min。结论 该方法具操作简易快速、定性准确、灵敏度高等特点, 能应用于应急检验, 为患者抢救工作节省了宝贵的时间。

英文摘要:

      Objective To establish a gas chromatography-mass spectrometry selected ion monitoring method for the determination of tetramine in the gastric content, the blood or the urine from the poisoned patients. Methods The C18-solid phase extraction column was adopt to purify the substrate of the gastric contents or the blood. Large volume injection of samples with a programmed temperature vaporize (PTV) system were discussed. With the conditions of small diameter capillary chromatographic column (HP-5MS: 20 m×0.18 mm, 0.18 μm), selected ion monitoring (SIM) acquired mode were selected for m/z 92, 132, 212, and 240. Results The whole process took about 20 min. Conclusion This method is rapid, accurate, and sensitive. It can be applied to emergency inspection, and save precious time for the patient in the tetramine poisoning rescue work.