肠侵袭性大肠埃希氏菌国家核酸标准样品的研制

Development of Enteroinvasive Escherichia coli nucleic acid reference material

中文关键词:  肠侵袭性大肠埃希氏菌  核酸标准样品  研制

英文关键词:Enteroinvasive Escherichia coli  nucleic acid reference material  research

中文摘要:

      目的 研制肠侵袭性大肠埃希氏菌核酸标准样品, 并对其均匀性和稳定性进行评价。方法 本文研究了肠侵袭性大肠埃希氏菌核酸标准样品的关键制备技术和保存技术, 定值技术和定值方式, 开展均匀性和稳定性试验, 并对食品中肠侵袭性大肠埃希氏菌核酸标准样品的不确定度进行确定；标准样品采用PicoGreen DNA分子荧光定量方法进行定值。多家单位合作定值, 确定标准样品的标准值及其不确定度。结果 标准样品均匀性标准不确定度为0.016 %, 不稳定标准差为0.0100, 均匀性和稳定性试验结果表明, 本文研制的标准样品均匀且稳定。结论 研制出具有溯源性的肠侵袭性大肠埃希氏菌核酸标准样品并获得国家标准样品证书, 可适用于肠侵袭性大肠埃希氏菌的检测和质量控制。

英文摘要:

      Objective Enteroinvasive Escherichia Coli(EIEC) Molecular DNA Reference Material was developed, and uniformity and stability of test were evaluated. Methods The key preparation technology, saving technology, certified technique and mode for EIEC Molecular DNA Reference Material were researched, via uniformity and stability test. The uncertainty for EIEC Molecular DNA Reference Material was evaluated in food. The standard value was determined by PicoGreen DNA molecule fluorescence quantitative methods. Standard and uncertainty of reference material was determined by many units cooperation. Results Uniformity standard uncertainty of reference material was 0.016 %, unstable standard deviation was 0.0100. The result of uniformity and stability test showed that reference material developed in this study was uniform and stable. Conclusion EIEC Molecular DNA Reference Material with the traceability was developed, and certificate of national reference material was obtained. It could be used for quantitative detection and qualitative quality control of EIEC.