15N稳定同位素标记集胞藻的生物合成研究

Research on biosynthese of stable isotope 15N labeled Synechocystis

中文关键词:  15N  稳定同位素  集胞藻  生物合成

英文关键词:15N  stable isotope  synechocystis  biosynthesis

中文摘要:

      目的 建立实验室生物合成15N稳定同位素标记集胞藻的最优培养条件, 并驯化得到高丰度15N集胞藻藻种。方法 对培养温度、光照及pH值等条件进行优化, 优选出最佳培养工艺。将培养基中的14N氮源替换为15N氮源, 优化配方, 使用同位素比质谱仪测量藻体中15N、14N同位素组成。结果 实验室培养条件选定为培养温度25 ℃、光照强度2000 lux、初始pH值为7时, 集胞藻生长良好。将配方中硝酸钠浓度设定为1.5 g/L时, 产品丰度最大。驯化得到高丰度15N-集胞藻藻种。结论 本试验优化的培养工艺和配方, 适宜15N稳定同位素标记集胞藻生长。得到的藻种产品丰度高, 生产成本低。

英文摘要:

      Objective To establish the optimal culture conditions of biosynthesis 15N stable isotope labeled Synechocystis, and to get high abundance 15N Synechocystis sp. strains. Methods The culture temperature, shine intensity and pH for Synechocystis sp. were studied in this paper. 15N nitrogen was used to replace 14N nitrogen in the medium. Isotope composition of 15N, 14N was measured by isotope ratio mass spectrometer (IRMS). Results The optimal medium conditions for Synechocystis sp. strain based on BG-11 medium were determined as follows: the NaNO3 concentration was 1.5 g/L (the rest of the components were the same as BG-11 medium). The optimal culture conditions were as follows: temperature of 25 ℃, light intensity of 2000 lux and initial pH of 7 in the room. Conclusion This paper built an academic foundation for achieving high density and amplificatory culture of Synechocystis sp. PCC6803.