基于SPME-GC-MS的四季桂花和枝叶挥发性 物质测定

Analysis of the volatile components from flowers and leaves of Osmanthus fragrans var. semperflorens by GC-MS combined with SPME

中文关键词:  固相微萃取法  气质联用法  四季桂花  挥发性物质

英文关键词:solid-phase microextraction  gas chromatography−mass spectrometry  Osmanthaus fragrans var. semperflorens  volatile components

中文摘要:

      目的 研究四季桂花的香气及枝叶的挥发性物质组成。方法 采用固相微萃取法富集四季桂花的香气及枝叶的挥发性成分, 通过气相色谱质谱联用技术测定挥发性物质组成。结果 结合质谱和保留指数分别从花和枝叶中鉴定出23和18种化合物, 占各自化合物总量的85.73%和64.58%。四季桂花的香气主要由牻牛儿酸乙酯、反式-香叶醇、香叶酸甲酯、反式-柠檬醛、顺式-柠檬醛、茶香螺烷及壬醛组成; 四季桂枝叶的鉴定的挥发性物质主要由酯类、醛类和碳氢化合物组成, 主要释放乙酸叶醇酯、甲氧基乙酸-2-十三烷基酯、4,11-二甲基-十四烷、苯乙醛和苯乙醇。结论 固相微萃取与气质联用技术便捷实用, 适用于测定桂花和枝叶挥发性物质, 从而为桂花资源开发利用、保护、及园林配置提供一定的理论依据。

英文摘要:

      Objective To characterize the volatiles in picked flowers and leaves of Osmanthus fragrans var. semperflorens, and to understand the volatile composition and content in them. Methods Head-space-solid-phase microextraction and gas chromatography- mass spectroscopy (GC-MS) were used to characterize the volatiles in picked flowers and leaves of Osmanthus fragrans var. semperflorens. Results A total of 23 components in the flowers and 18 in the leaves were identified by matching EI mass spectra and retention indices with the literature data, accounting for 85.73% and 64.58% total content of volatiles in flowers and leaves, respectively. Briefly, ethyl geranate, trans-geraniol, methyl geranate, trans-citral, 2,6,10,10-tetramethyl-1-oxa-spiro[4.5] dec-6-ene, cis-citral and nonanal were the most common volatiles in tested flowers. The major odors found in the leaves were cis-3-hexenyl acetate, 4,11-dimethyl- tetradecane, Methoxyacetic acid, 2-tridecyl ester, benzeneacetaldehyde and phenylethyl alcohol. Conclusion The method of HS-SPME/GC-MS is practical and suitable for the analysis of the volatile of picked flowers and leaves of Osmanthus fragrans var. semperflorens, so as to facilitate the development of Osmanthus scultivars to meet requirements of the food and fragrance industries.