间接酶联免疫法检测鲑鳟鱼类传染性 造血器官坏死病病毒

Identification of infectious hematopoietic necrosis virus in trout by indirect-enzyme-linked immune assays

中文关键词:  鲑鳟鱼  传染性造血器官坏死病病毒  病毒糖蛋白  间接酶联免疫法

英文关键词:trout  infectious hematopoietic necrosis virus  IHNV-G protein  indirect-enzyme-linked immune assays

中文摘要:

      目的　建立快速检测传染性造血器官坏死病病毒(IHNV)的间接酶联免疫法。方法　以传染性造血器官坏死病毒G蛋白(IHNV-G)为抗原, 免疫实验白兔, 制备兔抗IHNV免疫血清, 通过ELISA对兔抗血清进行效价及特异性评估, 并通过PCR方法进行验证。结果　应用间接ELISA 技术, 检测到抗血清对IHNV-G和IHNV的效价分别达到1:32000和1:16000, 与其他对照病毒无交叉反应。与PCR检测结果符合率达到98%。结论　本文建立的间接酶联免疫法方法具有良好的灵敏性和特异性, 为IHNV的检测提供了快捷、高效的技术手段。

英文摘要:

      Objective To establish an indirect enzyme-linked immune assays (ELISA) for the identification of infectious hematopoietic necrosis virus (IHNV). Methods An experimental rabbit was immunized to pro-duce anti-IHNV serum with IHNV-G protein. To assess the titer and specificity of the anti-serum, the ELISA test was used. Further, PCR was used to verify the above ELISA experimental result. Results The titer of anti-serum of IHNV-G and IHNV reached to 1:32000 and 1:16000, respectively, moreover, the cross-reaction with other virus was 0%, and ELISA results was with 98% accordance rate with the method of PCR. Conclusion The indirect-ELISA of this research has a good specificity and sensitivity, and it is suitable for rapid detection and epidemiological investigation of IHNV infection in trout.