高效液相色谱法测定不同产地连翘花中 四种有效成分含量

Determination of four components in Forsythia suspensa flower from different habitats by high performance liquid chromatography

中文关键词:  连翘花  高效液相色谱法  芦丁  连翘酯苷A  连翘苷  松酯酚

英文关键词:Forsythia suspensa flower  high performance liquid chromatography  rutin  forsythoside A  forsythin  pinoresinol

中文摘要:

      目的　建立测定不同产地连翘花中芦丁、连翘酯苷A、连翘苷、松酯酚含量的高效液相色谱法, 为其有效成分控制提供有效方法。方法　采用Symmetry C18(4.6 mm×150 mm, 5 μm)色谱柱, 流动相为乙腈-水, 梯度洗脱, 流速1.0 mL/min, 检测波长230 nm, 柱温25 ℃。结果　不同产地的连翘花之间各成分的含量差异显著, 其中连翘酯苷A在各样品中含量最高, 芦丁含量次之, 连翘苷含量最少; 其中菏泽产的样品连翘酯苷A含量高达119.38±5.78 mg/g, 连翘苷的含量达4.71±0.09 mg/g; 临沂产的样品芦丁含量最高达75.64±2.09 mg/g; 日照、青岛和烟台的样品中松脂酚含量较高且相差不大, 分别是25.41±1.51、24.45±0.98、23.60±1.19 mg/g。结论 12个产地连翘花中连翘酯苷A的含量远远高于药典规定的最低含量要求。但连翘花中连翘苷的含量较低。

英文摘要:

      Objective To establish a method for determination of rutin, forsythoside A, forsythin and pinoresinol in different regions of Forsythia suspensa flower by high performance liquid chromatography, thus to offer a method for its quality control. Methods Symmetry C18 (4.6 mm×150 mm, 5 μm) column was adopted. The mobile phase was acetonitrile-water with gradient elution at the flow rate of 1.0 mL/min. The detection wavelength was 230 nm, and the column temperature was 25 ℃. Results The contents of rutin, forsythoside A, forsythin and pinoresinol in different regions of F. suspensa flower were significantly different, and the contents of forsythoside A in the F. suspensa flower was the highest, then followed by rutin and forsythin. Among them, the content of forsythoside A and forsythin of the F. suspensa flower in region of Heze were 119.38±5.78 mg/g and 4.71±0.09 mg/g, respectively. The content of rutin of the F. suspensa flower in region of Linyi was 75.64±2.09 mg/g, which was the highest. High content of pinoresinol in regions of Rizhao, Qingdao and Yantai are 25.41±1.51, 24.45±0.98 and 23.60±1.19 mg/g, respectively, which showed no significant difference. Conclusion Although the content of forsythin of F. suspensa flower was low, the content of forsythoside A in 12 regions was significantly higher than that of the Chinese Pharmacopoeia requirement.