高效液相色谱法测定食品中双乙酸钠和 丙酸盐含量

Determination of sodium diacetate and propionate in food by HPLC

中文关键词:  食品  双乙酸钠  丙酸  高效液相色谱法

英文关键词:food  sodium diacetate  propionate  HPLC

中文摘要:

      目的 建立食品中同时测定双乙酸钠和丙酸钠(钙)的高效液相色谱检测方法。方法 样品中的双乙酸钠、丙酸钠(钙)经(1 1)盐酸溶液酸化后转化为乙酸与丙酸, 使用有机溶剂进行萃取后在40 ℃下水浴挥干, 浓缩后使用流动相定容, 经C18色谱柱分离, 在215 nm波长处测定, 外标法定量。结果 在优化的实验条件下, 双乙酸钠和丙酸钠(钙)在0.5~5.0 mg/mL范围内线性关系良好, 相关系数均大于0.9998, 方法的检出限为2 mg/kg, 加标回收率在87.3%~100.6%范围之内, 相对标准偏差在0.89%~4.53%之间。结论 该方法前处理简便快速, 定量准确可靠, 灵敏度高, 适用于各类食品中双乙酸钠、丙酸钠(钙)含量的同时测定。

英文摘要:

      Objective To set up a method for quantitative detection of sodium diacetate and propionate in food by HPLC. Methods Sodium diacetate and propionate in samples were converted into acetic acid, propionic acid after acidated with (1 1) HCl and extracted by organic solvent. The extracts were blown to dryness at 40 ℃ and made up volume after concentration with mobile phase. The chromatographic separation was performed on a C18 column and detected by the UV detector at a wavelength of 215 nm. Results Under the optimal conditions, the method showed a good linearity in the range of 0.5~5.0 mg/mL with correlation coefficient more than 0.9998. The detection limit was 2 mg/kg, however the spiked recoveries were between 87.3%~100.6%, and the RSD was in the range of 0.89%~4.53%. Conclusion The method is simple, accurate and sensitive, it is suitable for the determination of batch food samples.