果胶与双歧杆菌对Balb/c小鼠机体敏感性的影响

Research on the effect of pectin and Bifidobacterium on organism sensibility in Balb/c mice

中文关键词:  果胶  双歧杆菌  小鼠  过敏反应  肠道菌群

英文关键词:pectin  Bifidobacterium  mice  anaphylaxis  intestinal flora

中文摘要:

      目的 研究果胶是否能够降低Balb/c小鼠对OVA(鸡卵清白蛋白)的过敏反应水平, 以及同时经口给予双歧杆菌是否能够调整小鼠肠道菌群, 从而影响过敏反应的发生和反应强度。方法 连续40 d经口给予小鼠20 μg/只(低剂量)和200 μg/只(高剂量)的果胶溶液或溶有双歧杆菌(2?106 CFU/只)的果胶溶液, 每10 d腹腔注射OVA和铝佐剂致敏小鼠, 10 d后进行大刺激, 采血测定组胺、肥大细胞蛋白酶-1、免疫球蛋白E水平, 并对脾脏T细胞进行体外刺激, 检测IFN-γ和IL-4的水平, 同时取小鼠粪便筛选双歧杆菌培养。结果 果胶处理组小鼠血浆中组胺、肥大细胞蛋白酶-1水平与OVA致敏组相比无显著差异(P>0.05); 高剂量处理组小鼠血清总IgE水平显著低于OVA致敏组(P<0.05), 特异性IgE及细胞因子水平无显著差异(P>0.05); 双歧杆菌筛选培养可以看出, 果胶有增加双歧杆菌数量的作用, 但经口给予双歧杆菌活菌无显著作用。结论 补充果胶不能降低Balb/c小鼠的肥大细胞脱颗粒情况、抗体水平以及细胞因子产生水平; 果胶有增加肠道内双歧杆菌的作用, 但经口给予活菌不能增加肠道内双歧杆菌水平。

英文摘要:

      Objective To study whether pectin can decrease the level of allergic reactions to ovalbumin ( OVA ) in Balb/c mice, and whether oral administration of bifidobacterium at the same time can adjust the intestinal flora in mice to affect the level of an allergic reaction. Methods 20 μg/mouse (low dose) and 200 μg/mouse (high dose) soluble pectin solution with/without Bifidobacterium (2?106 CFU/mouse) was given for 40 d, and OVA added alum adjuvant was intraperitoneally injected every 10 days to sensitize mice, then mice were stimulated with OVA 10 d later. The levels of plasma histamine, mast cells protease-1 and immunoglobulin E were determined by ELISA. Spleen T cells were prepared and stimulated in vitro to produce cytokines, and the levels of IFN-gamma and IL–4 were then measured. Fecal samples were collected to culture Bifidobacterium, and compare the differences between groups. Results The levels of histamine, mast cells protease-1 level of mice administrated with pectin and mice sensitized by OVA showed no significant difference. Levels of serum total IgE in mice of high dose treatment were significantly lower than mice sensitized by OVA, while the levels of specific IgE and cytokines showed no significant difference. Pectin could increase the number of bifidobacteria, but oral administration of bifidobacterium viable showed no significant effect. Conclusion Levels of mast cell degranulation, antibodies and cytokines were not reduced by pectin in Balb/c mice. Pectin increased the number of intestinal Bifidobacterium, but it showed no obvious effect by given live bacteria.