禽畜肉中利巴韦林残留量检测方法的研究

Study on detection methods of ribavirin in tissues of livestock and poultry

中文关键词:  利巴韦林  液相色谱-串联质谱法  固相萃取  禽畜肉

英文关键词:ribavirin  liquid chromatography-tandem mass spectrometry  solid phase extraction  tissues of livestock and poultry

中文摘要:

      .目的 建立禽畜肉中利巴韦林的高效液相色谱-串联质谱(HPLC-MS/MS)检测方法。方法 待测样品中的利巴韦林用0.25 mol/L醋酸铵缓冲溶液(pH 4.8)提取, 经固相萃取柱净化、浓缩、定容后采用液相色谱-串联质谱法在正离子模式下检测。结果 该方法在10~300 μg/kg范围内线性关系良好, 相关系数r>0.999。利巴韦林在禽肉及畜肉中的检测限均为10 μg/kg, 回收率在97.0%~109.4%之间, 精密度(以相对标准偏差表示)在0.7%~2.8%之间(n=6)。结论 本方法具有前处理简单、选择性好、灵敏度高等优点, 可用于禽畜肉中利巴韦林残留量的定量检测。

英文摘要:

      Objectives To establish a method for the determination of ribavirin in tissues of livestock and poultry by high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Methods The residues of ribavirin in the samples were extracted with buffer (ammonium acetate pH 4.8). After being cleaned up with SPE column, concentrated and reconstituted, the residues were detected by HPLC-MS/MS under multiple reaction monitoring (MRM) via positive ionisation mode. Results The limit of quantification was 10 μg/kg. The method showed a good linearity over the range of 10~300 μg/kg for ribavirin with r>0.999. The average recoveries at 3 spiked concentration levels were between 97.0%~109.4%, while the relative standard deviations (RSD) were between 0.7%~2.8% (n=6). Conclusion The established method is highly sensitive, accurate and reproducible. It is suitable for the detection of ribavirin in tissues of livestock and poultry