液相色谱-串联质谱基质效应及其消除方法

Matrix effects and elimination methods of liquid chromatography-tandem mass spectrometry

中文关键词:  液相色谱-串联质谱  基质效应  消除方法

英文关键词:liquid chromatography-tandem mass spectrometry  matrix effect  elimination methods

中文摘要:

      综合近年来国内外进行液相色谱-串联质谱方法开发中关于基质效应的相关报道, 重点介绍了液相色谱-串联质谱基质效应的消除方法, 主要包括: 选择合适的样品前处理方法; 选择最佳的色谱分离条件; 优化的质谱分析参数; 使用恰当的内标; 采用基质标准溶液校正。这些方法的应用, 可有效改善基质效应的影响, 优化液相色谱-串联质谱检测方法。

英文摘要:

      The related domestic and international reports on the matrix effects of liquid chromatography-tandem mass spectrometry (LC-MS/MS) method were reviewed. The elimination methods of LC-MS/MS matrix effects were introduced, such as selection of appropriate method for sample preparation, selection of suitable chromatographic separation conditions, optimization of conditions for mass spectrometry, usage of suitable internal standard, calibration by matrix matched standard solution. These methods can effectively improve the effect of matrix effect, optimize the method of liquid chromatography tandem mass spectrometry.