豆制品和粉干中乌洛托品的气质联用测定

Determination of urotropine in soybean products and cereal noodles by gas chromatography-mass spectrometry

中文关键词:  乌洛托品  气质联用(GC-MS)  豆制品  粉干

英文关键词:urotropine  gas chromatography-mass spectrometry (GC-MS)  soybean products  cereal noodles

中文摘要:

      目的 建立豆制品(如腐竹、豆腐干等)和粉干中乌洛托品的气质联用(GC-MS)测定方法。方法 样品中乌洛托品经三氯乙酸水溶液提取、阳离子交换柱净化后GC-MS测定。 结果 该方法的线性范围为0.1~5 mg/kg, 相关系数r为0.998; 检测限为0.05 mg/kg; 回收率为78.5%~97.0%; 相对标准偏差(RSD)为5.7%~10.9%。结论 该方法准确、操作简单, 可用于实际样品中乌洛托品的含量测定。

英文摘要:

      Objective To establish a qualitative and quantitative method for the determination of urotropine in soybean products and cereal noodles by gas chromatography-mass spectrometry (GC-MS). Methods The sample was extracted by 1.5% trichloroacetic acid aqueous solution. After purified by cation exchange cartridge, the sample solution was detected by GC-MS. Results A calibration curve ranging from 0.1 to 5 mg/kg of urotropine was used to establish instrument response with relative coefficient r＝0.998. The recovery was 78.5%~97.0% with relative standard deviation (RSD) 5.7% to 10.9%. The limit of detection was 0.05 mg/kg. Conclusion The method is accurate and easy to operate. It is suitable for the determination of urotropine in real sample.