环太湖水域微囊藻毒素-LR污染状况检测研究

Study on microcystins-LR pollution situation of Taihu rim

中文关键词:  微囊藻毒素-LR  环太湖  时间分辨荧光免疫分析法(TRFIA)

英文关键词:microcystins-LR  the Taihu lake rim  time-resolved fluorescence immunoassay analysis

中文摘要:

      目的 了解环太湖沿岸主要城市生活饮用水中微囊藻毒素-LR的污染状况, 建立环太湖水源水及无锡、苏州和湖州自来水厂出厂水中MC-LR浓度的时间分辨荧光免疫分析方法(TRFIA)。方法 将偶联牛血清白蛋白的MC-LR包被96孔板, 密封抽真空后, 加入MC-LR标准溶液或样品, 再加入抗MC-LR兔血清后用全自动TRFIA检测仪测量, 通过标准曲线计算样品中MC-LR的含量。结果 环太湖水源水MC-LR检出率为60.0%, 平均浓度仅0.390 μg/L, 自来水厂出厂水检出率为28.8%, 其中全年检出最大量1.95 μg/L。结论 太湖水存在藻毒素污染的问题, 但是水质符合国家标准。

英文摘要:

      Objective To investigate the drinking water pollution state of microcystins-LR (MC-LR) in major cities around Tai lake and establish a method for the determination of MC-LR of source water and tap water in the city of Wuxi, Suzhou and Huzhou around the Taihuby time-resolved fluorescence immunoassay analysis (TRFIA) . Methods The MC-LR coupled with bovine serum albumin was coated into the 96 wells plate, vacuum sealed , added with MC-LR standard solutions or samplesand then was added with an-ti-MC-LR rabbit serum . After that, thecontent of MC-LR was detected through automatic TRFIA detector and calculated by the standard curve. Results The concentration of MC-LR in source water in Tai lake was only 0.390 μg/L in average, and 1.95 μg/L in maxium for the whole year, and the positive rate of source water and tap water were 60.0% and 28.8%, respectively. Conclusion The Tai lake water has MC-LR pollution problems, but it is accorded with national standard.