反相高压液相色谱法测定蜂胶保健食品中白杨素、高良姜素的含量

Determination of chrysin and galangin in propolis health foods by reversed-phase high pressure liquid chromatography

中文关键词:  白杨素  高良姜素  反相高压液相色谱法  保健食品

英文关键词:chrysin  galangin  reversed-phase high pressure liquid chromatography  health care foods

中文摘要:

      目的 建立用反相高压液相色谱测定保健食品中白杨素、高良姜素的方法。方法 蜂胶样品中白杨素、高良姜素经甲醇水浴回流提取、C18反相柱分离, 在波长268 nm处检测。结果 此方法得到的白杨素回收率为95.5%~100.8%, RSD为1.1%~2.0%, 高良姜素回收率为95.2%~100.5%, RSD为1.6%~2.8%。结论 此方法简便快速, 准确可靠, 可用于对一些蜂胶保健食品中白杨素、高良姜素含量的测定。

英文摘要:

      Objective To establish a method for the detection of chrysin and galangin in propolis health care foods by reversed-phase high pressure liquid chromatography (RP-HPLC). Methods Chrysin and galangin in samples were reflux extracted with methanol in water bath, separated by C18 reversed phase column, and detected at wavelength of 268 nm. Results The recovery of chrysin was 95.6%~100.8% with the relative standard deviations of 1.1%~2.0%, and the recovery of galangin was 95.2%~100.5% with the relative standard deviations of 1.6%~2.8%. Conclusion The method is simple, rapid, accurate and reliable, which could be used for the detection of chrysin and galangin in propolis health care foods.