鱼体中两种挥发性有机残留物的提取与检测

The extraction and detection of two volatile organic residues in fish

中文关键词:  鱼  挥发酚  硝基苯  水蒸汽蒸馏法  提取  残留检测

英文关键词:fish  volatile phenol  nitrobenzene  water steam distillation  extraction  residue detection

中文摘要:

      目的 研究水蒸汽蒸馏方法提取鱼体中挥发酚和硝基苯的效果, 并研究鱼体中挥发酚和硝基苯残留量的测定方法。方法 用自制水蒸汽发生器蒸馏提取挥发酚和硝基苯, 并测定两种物质含量。结果 水蒸汽发生器蒸馏法泡沫产生少, 蒸馏过程稳定、容易控制, 不存在暴沸现象。鱼体中挥发酚测定平均回收率在85.5%以上, 硝基苯测定平均回收率为96.83%。硝基苯出峰时间为10.051 min, 分离度高, 检测时间短、消耗有机溶剂量少、试验简便, 完全能够满足检测分析的需要。 结论 水蒸汽蒸馏提取法效果好, 方法实用, 便于操作, 适用于处理后样品的分析测定, 已应用于鱼体中挥发酚和硝基苯残留量的测定。

英文摘要:

      Objective To investigate the effect of the steam distillation on extracting volatile phenol and nitrobenzene in fish and explore methods for detecting their residues. Methods A homemade steam generator was used to distillate and extract volatile phenol and nitrobenzene, and the content of the two kinds of material were determined. Results Steam generator distillation produced few bubbles, the distillation process was stable, easy to control, and free of sudden boiling. The average recovery of volatile phenol in the fish body was more than 85.5% and that of nitrobenzene was 96.83%. The peak time for nitrobenzene was 10.051 min, with a high separating degree and short testing time. The consumption of organic solvent for the method was little, the testing was simple, which could completely meet the needs of inspection and analysis. Conclusion Steam distillation extraction is effective, practical, easy for operation, and suitable for the analysis of the prepared samples, which has been utilized in the determination of nitrobenzene and volatile phenol residues in the fish body.