紫外光度法测定蜂蜜中残留土霉素和盐酸金霉素的含量

Determination of oxytetracycline and chlortetracycline hydrochloride in honey by ultraviolet spectrophotometry

中文关键词:  蜂蜜  抗生素  药物残留  紫外光度法

英文关键词:honey  antibiotic  drug residue  ultraviolet spectrophotometry

中文摘要:

      目的 建立测定蜂蜜中土霉素和盐酸金霉素含量的方法。方法 采用紫外光度法测定土霉素和盐酸金霉素的含量。结果 在甲醇溶液中, 土霉素和盐酸金霉素的最大吸收波长分别在364 nm和319 nm处, 在0.56~6.72 ?g/mL和1.03~20.60 ?g/mL浓度范围内有很好的线性关系, 回归方程和相关系数分别为Y= 0.0254X?0.00644, r=0.99355和Y=0.02391 0.02756X, r=0.99493。结论 该方法操作简单, 重现性好, 可准确用于蜂蜜样品中土霉素和盐酸金霉素含量的测定, 结果满意。

英文摘要:

      Objective To establish a method for determining the content of oxytetracycline and chlortetracycline hydrochloride of honey samples. Methods The content of oxytetracycline and the chlortetracycline hydrochloride were absorbed by ultraviolet (UV) spectrophotometry. Results In methanol solution, the maximum absorption wavelengths of oxytetracycline and the chlortetracycline hydrochloride were at 364 nm and 319 nm, respectively. It had a good linear relationship between the range of 0.56~6.72 ug/mL and 1.03~20.60 ug/mL concentration, and the regression equation and the correlation coefficient were Y=0.0254X? 0.00644, r=0.99355 and Y=0.02391 0.02756X, r=0.99493. Conclusion The method has the advantages of simple operation, good reproducibility, and accuracy, and it can be used to determine the content of oxytetracycline and chlortetracycline hydrochloride of honey samples.