沙门氏菌的依赖于核酸序列恒温扩增检测方法的建立

Establishment of the nucleic acid sequence-based amplification method of detecting Salmonella

中文关键词:  沙门氏菌  检测  依赖于核酸序列恒温扩增  

英文关键词:salmonella  detection  nucleic acid sequence-based amplification

中文摘要:

      采用自行建立和优化的依赖于核酸序列恒温扩增(nucleic acid sequence-based amplification,NASBA)检测体系,对沙门氏菌进行检测。采用沙门氏菌的invA基因为目的片段设计特异性引物,建成可快速检测沙门氏菌的NAS-BA检测法,并进行了特异性和灵敏度试验。结果表明:所建立起的NASBA检测方法,灵敏度为7.1×102cfu/ml,高于普通PCR方法。

英文摘要:

      A new method,which based on Nucleic Acid Sequence-based Amplification(NASBA) to detect Salmonella of sample,was established.A specific set of primers was synthesized to target the invA gene of Salmonella so as to establish Nucleic Acid Sequence-based Amplification method.Specific and sensitivity were tested.The results shows that the sensitivity of NASBA was 7.1×102 cfu/ml which was higher than the result of PCR method.Detecting Salmonella with NASBA was more specific and sensitive than PCR method and has l...