姓名：张显

性别：男

学历：博士研究生

职称：副教授

工作单位：江南大学生物工程学院

教育情况：

2007.09~2014.01   江南大学，发酵工程专业，工学博士

2003.09~2007.06   江南大学，生物科学专业，理学学士

工作经历：

2017.06~至  今   江南大学，生物工程学院，硕士生导师

2016.09~至  今   江南大学，生物工程学院，副教授

2014.03~2016.08   江南大学，生物工程学院，讲师

其中：

2018.10~2019.10   美国俄亥俄州立大学，化学与生化分子工程系，访问学者

所受奖项：

中国轻工业联合会科学技术发明二等奖，2016

中国商业联合会科技进步特等奖，2018

江苏省科技进步二等奖，2018

主要研究方向：

主要从事发酵微生物选育、工业微生物代谢工程育种、工业/食品用酶开发等研究，近年来在微生物发酵法生产四碳基平台化合物、生物催化法生产高值氨基酸、工业/食品用酶改良等方面开展多项工作。合作发表SCI论文30余篇（第一作者在Metabolic Engineering，Amino Acids等国际权威期刊发表SCI论文8篇，共同第一作者SCI论文2篇，单篇最高IF值>8.0）。近5年来主持863计划子课题、国家自然科学基金、江苏省自然科学基金、中国博士后面上项目和特别资助项目5项。参与申报发明专利30余项（其中申报国际发明专利9项），获授权中国发明专利10余项。

代表性著作：

发表的代表性SCI文章（?通讯作者；Δ共同第一作者）

1.      Zhang XianΔ, Zhang RongzhenΔ, Bao Teng, Rao Zhiming?, Yang Taowei, Xu Meijuan, Xu Zhenghong, Li Huazhong?, Yang Shangtian. The rebalanced pathway significantly enhances acetoin production by disruption of acetoin reductase gene and moderate-expression of a new water-forming NADH oxidase in Bacillus subtilis [J]. Metabolic Engineering, 23: 34-41, 2014.

2.      Zhang Xian, Rao Zhiming*, Li Jingjing, Zhou Junping, Yang Taowei, Xu Meijuan, Bao Teng, Zhao Xiaojing. Improving the acidic stability of Staphylococcus aureus α acetolactate decarboxylase in Bacillus subtilis by changing basic residues to acidic residues [J]. Amino Acids, 47(4):707–717, 2015.

3.      Zhang XianΔ, Bao TengΔ, Rao Zhiming?, Yang Taowei, Xu Zhenghong, Yang Shangtian, Li Huazhong?. Two-stage pH Control Strategy Based on The pH Preference of Acetoin Reductase Regulates Acetoin and 2,3-butanediol Distribution in Bacillus subtilis [J]. PLoS One, 9(3): e91187, 2014.

4.      Zhang Xian, Zhang Rongzhen*, Bao Teng, Yang Taowei, Xu Meijuan, Li Huazhong, Xu Zhenghong, Rao Zhiming*. Moderate expression of the transcriptional regulator ALsR enhances acetoin production by Bacillus subtilis [J]. J Ind Microbiol Biotechnol, 40(9):1067-1076, 2013.

5.      Zhang Xian, Yang Taowei, Lin Qing, Xu Meijuan, Xia Haifeng, Xu Zhenghong, Li Huazhong, Rao Zhiming*. Isolation and identification of an acetoin high production bacterium that can reverse transform 2,3-butanediol to acetoin at the decline phase of fermentation [J]. World J Microbiol Biotechnol, 27(12):2785-2790, 2011.

6.      Zhang Xian, Zhang Rongzhen*, Yang Taowei, Zhang Jing, Xu Meijuan, Li Huazhong, Xu Zhenghong, Rao Zhiming*. Mutation breeding of acetoin high producing Bacillus subtilis blocked in 2,3-butanediol dehydrogenase [J]. World J Microbiol Biotechnol, 29: 1783-1789, 2013.

7.      Xian Zhang, Zhiming* R, Zhang L, Meijuan X, Taowei Y. Efficient 9α-hydroxy-4-androstene-3,17-dione production by engineered Bacillus subtilis co-expressing Mycobacterium neoaurum 3-ketosteroid 9α-hydroxylase and B. subtilis glucose 1-dehydrogenase with NADH regeneration. SpringerPlus 5:1207, 2016.

8.      Xian Zhang,  Dan Wu, Taowei Yang, Meijuan Xu, Zhiming Rao*. Over-expression of Mycobacterium neoaurum 3-ketosteroid-Δ1-dehydrogenase in Corynebacterium crenatum for efficient bioconversion of 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione. Electronic Journal of Biotechnology, 2016, 24: 84-90.

9.      Teng BaoΔ, Xian ZhangΔ, Xiaojing Zhao, Zhiming Rao?, Taowei Yang，Shangtian Yang. Regulation of the NADH pool and NADH/NADPH ratio redistributes acetoin and 2,3-butanediol proportion in Bacillus subtilis. Biotechnology Journal, 8(10): 1298-1306, 2015.

10.  Bao TengΔ, Zhang XianΔ, Rao Zhiming?, Zhao Xiaojing, Zhang Rongzhen, Yang Taowei, Xu Zhenghong, Yang Shangtian. Efficient whole-cell biocatalyst for acetoin production with NAD+ regeneration system through homologous co-expression of 2,3-butanediol dehydrogenase and NADH oxidase in engineered Bacillus subtilis. PLoS One, 9(7): e102951, 2014.

11.  Samuel Niyomukiza, Bao Teng, Zhang Xian?, Yang Taowei, Xu Meijuan, Li Xin, Komera Irene, Philibert Tuyishime, Rao Zhiming?. Optimized whole cell biocatalyst fromacetoin to 2,3-butanediol through coexpression of acetoin reductase with NADH regeneration systems in engineered Bacillus subtilis. J Chem Technol Biotechnol, 2017, 92(9):2477-2487.

12.  Xu Li, Xian Zhang?, Shuqin Xu, Hengwei Zhang, Meijuan Xu, Taowei Yang, Li Wang, Haifeng Qian, Huiling Zhang, Haitian Fang, Tolbert Osire, Zhiming Rao?, Shangtian Yang. Simultaneous cell disruption and semi-quantitative activity assays for high-throughput screening of thermostable L-asparaginases. Scientific Reports, 2018, 8:7915.

13.  Minglong Shao, Xian Zhang, Zhiming Rao*, Meijuan Xu, Taowei Yang, Hui Li, Zhenghong Xu, Shangtian Yang. Efficient testosterone production by engineered Pichia pastoris co-expressing human 17β-hydroxysteroid dehydrogenase type 3 and Saccharomyces cerevisiae glucose 6-phosphate dehydrogenase with NADPH regeneration.Green Chemistry, 2016, 18:1774-1784.

14.  Rongzhen Zhang, Taowei Yang, Zhiming Rao*, Hongmei Sun, Meijuan Xu, Xian Zhang, Zhenghong Xu, Shangtian Yang. Efficient one-step Preparation of γ-Aminobutyric Acid from Glucose without Exogenous Cofactor by the Designed Corynebacterium glutamicum. Green Chemistry. 2014, 16: 4190-4197.

15.  Man Zaiwei, Zhiming Rao*, Xu Meijuan, Guo Jing, Yang Taowei, Zhang Xian, Xu Zhenghong*. Improvement of the intracellular environment for enhancing L-arginine production of Corynebacterium glutamicum by inactivation of H2O2-forming flavin reductases and optimization of ATP supply. Metabolic Engineering. 2016, 38:310.

16.  Yang Taowei, Rao Zhiming*, Hu Guiyuan, Zhang Xian, Liu Mei, Dai Yue, Xu Meijuan, Xu Zhenghong, Yang Shangtian. Metabolic engineering of Bacillus subtilis for redistributing the carbon flux to 2,3-butanediol by manipulating NADH levels. Biotechnology for Biofuels, 8:129, 2015.

17.  Taowei Yang, Zhiming Rao*, Xian Zhang, Meijuan Xu, Zhenghong Xu, Shang-Tian Yang. Metabolic engineering strategies for acetoin and 2,3-butanediol production: Advances and prospects, Critical Reviews in Biotechnology, 2017, 37(8): 990-1005