姓名:王健

性别:

学历:博士

职称:教授

工作单位:吉林大学生物与农业工程学院生物工程系

 

教育情况:

19949月至19987月,吉林农业大学学士

19989月至20017月,吉林农业大学硕士

20019月至20049月,天津科技大学发酵工程专业博士

 

工作经历:

20049月 至20089月,吉林大学生物与农业工程学院生物工程系 讲师

20079月 至200810月,美国莱斯大学 访问学者

20089月至20149月,吉林大学生物与农业工程学院生物工程系 副教授

20149月至今,吉林大学生物与农业工程学院生物工程系 教授

主要研究方向:

长期从事氨基酸代谢工程、代谢控制发酵的科研与教学工作。特别是对氨基酸及核酸类物质代谢网络定量分析等方面进行深入研究,并以此为指导进行氨基酸及核酸类物质产生菌的定向改造和发酵过程的优化控制。

代表性著作:

著作:

[1]王健,怀丽华,周昌平,陈宁.酶工程. 北京:中国轻工业出版社. 2005    ISBN 7-5019-4979-4

[2]杜军,王健,王晋.氨基酸工业发展报告.北京:清华大学出版社.2011 ISBN978-7-302-27397-4

[3]宋存江,方柏山,王健等。发酵工程原理与技术. 北京:高等教育出版社.2014 ISBN 978-7-04-038233-4

已发表论文:

[1] Wang Jian, et al. Succinate production from different carbon sources under anaerobic conditions by metabolic engineered Escherichia coli strains. Metabolic Engineering. 2011.13(3): 328–335

[2] Wang Jian, et al. Succinate production from sucrose by metabolic engineered Escherichia coli strains under aerobic conditions. Biotechnology Progress. 2011.27(5):1242-1247

[3] Wang Jian, et al. Fermentation characterization of an L-tryptophan producing Escherichia coli strain with inactivated phosphotransacetylase. Ann Microbiol. 2013.63(4): 1219–1224

[4] Wang Jian, et al. Genetic engineering of Escherichia coli to enhance production of L-tryptophan. Applied Microbiology Biotechnology. 2013.97(17): 7587-7596.

[5] Wang Jian, et al. Mutagentic study of a novel inosine monophosphate dehydro-

genase from Bacillus amyloliquefaciens and its possible application in guanosine production. Biotechnology Biotechnological Equipment. 2014. 28:1, 102-106

[6] Wang Jian, et al. High-level production of L-threonine by recombinant Escherichia coli with combined feeding strategies. Biotechnology Biotechnological Equipment. 2014. 28:3, 495-501

[7] Wang Jian, et al. Optimization of Carbon Source and Glucose Feeding Strategy for Improvement of L-isoleucine Production by Escherichia coli. Biotechnol Biotechnol Equip. 2015.29(2):374-380 DOI: 10.1080/13102818.2015.1006899

[8] Wang Jian, et al. Impacts of Sodium Citrate on Metabolic Flux Distributions of L-valine Fermentation. CMBB. 2010

[9] Wang Jian, et al. Co-expression of threonine dehydratase and acetolactate synthase in Escherichia coli for L-isoleucine production. Review Form of 2014 4rd International Symposium on Chemical Engineering and Material Properties (ISCEMP 2014)

[10] Wang Jian, et al. Efficient Electroporation of Bacillus Amyloliquefaciens by Surmounting the Restriction Barrier 2014 International Conference on Biological Engineering and Biomedical (BEAB 2014)

[11] Cheng L.K., Wang J., et al. Effect of Feeding Strategy on L-Tryptophan Production by Recombinant Escherichia coli. Ann. Microbiol.2012. 62:1625-1634

[12] Cheng L.K., Wang J., et al. Strategy for pH control and pH feedback-controlled substrate feeding for high-level production of L-tryptophan by Escherichia coli. World Journal of Microbiology and Biotechnology. 2013.29(5): 883-890

[13] Wang Jing, Wen Bing, Wang Jian et al. Enhancing L-Isoleucine Production by thrABC Overexpression Combined with alaT Deletion in Corynebacterium glutamicum. Appl Biochem Biotechnol. 2013. 171(1): 20-30.