Name:HUANG Jin-Lin
Gender: Male
Degree: Ph.D
Title: Professor
Organization: College ofBioscience and Biotechnology, Yangzhou University
Education Experience:
2005-2008 Ph.D. Preventive Veterinary Medicine, Yangzhou University, China
1999-2002 M.S. Preventive Veterinary Medicine, Yangzhou University, China
1990-1994 B.S. Food hygiene, Yangzhou University, China
Work Experience:
2013-nowProfessor. College ofBioscience and Biotechnology, Yangzhou University
2007-2012 Associate Professor. College ofBioscience and Biotechnology, Yangzhou University
2001-2007 Lecturer. College ofBioscience and Biotechnology, Yangzhou University
1994-2000 Teaching Assistant. Department ofScience, Yangzhou University
Rewards:
Research Area:
l Epidemiological survey of pathogens of zoonoses
- Development of multiplex PCR for detection of Campylobacter spp
- Application of PCR to test Salmonella Mab diagnostic kit and for rapid detection of Salmonella
- Establishment and application of multiple PCR technique for detection of Salmonella spp. and Listeria monocytogenes
- Epidemiological survey of Campylobacter spp in diarrhea patients, dairy cattle, chickens, Waterfowl and food in China
- Antibiotic resistance of C. jejuni and C. coli isolated from humans, chickens and cattle
l molecular subtyping of pathogens of zoonoses
- Genotyping of C. jejuni isolates from different origins using PCR-RAPD, PCR-fla-RFLP and PFGE subtyping methods
- Genotyping of C. jejuni isolates from different origins using MLST method
l Molecular Mechanisms on Pathogenesis of C. jejuni
- Analysis of virulence-associated genes in C. jejuni and C. coli isolates
- Establishment and application of a C. jejuni infection model on HT-29 cell line
- Prokaryotic expression of C. jejuni cjaA and flaA in recombinant E.coli
- The successful preparation of mAbs specific for protein FlaA and CjaA of C. jejuni
l C. jejuni control strategies
- Explored a prophylactic strategy using chitosan-DNA intranasal immunization to induce specific immune responses against C. jejuni.
- Chitosan-pCAGGS-flaA intranasal immunization induced 2-3 log10 and 2 log10 reductions in large intestine and cecum of chickens, respectively, against homologous strain challenge, and a significant reduction of bacterial load after acute infection.
Revealed the inactivation kinetics characteristics for three C. jejuni strains on the chicken meat during the low-temperature storage and provided useful information for C. jejuni risk management.
Publication:
1) Huang J, Jiang F, Hu Y, Zhou X, Gu S, Jiao XA. An inactivation kinetics model for Campylobacter jejuni on chicken meat under low-temperature storage. Foodborne Pathog Dis, 2012, 9(6):513-516. （IF 2.262）
2) Huang J, Yang G, Meng W, Wu L, Zhu A, Jiao X. An electrochemical impedimetric immunosensor for label-free detection of Campylobacter jejuni in diarrhea patients' stool based on O-carboxymethylchitosan surface modified Fe3O4 nanoparticles. Biosens Bioelectron. 2010，25(5):1204-1211.（IF 5.429）
3) Huang JL, Yin YX, Pan ZM, Zhang G, Zhu AP, Liu XF, Jiao XA. Intranasal immunization with chitosan/pCAGGS-flaA nanoparticles inhibits Campylobacter jejuni in a White Leghorn model. J Biomed Biotechnol, 2010 (2010): ID 589476.
4) Huang JL, Xu HY, Bao GY, Zhou XH, Ji DJ, Zhang G, Liu PH, Jiang F, Pan ZM, Liu XF, Jiao XA. Epidemiological surveillance of Campylobacter jejuni in chicken, dairy cattle and diarrhoea patients. Epidemiol Infect, 2009, 137(8): 1111-1120.（IF 2.365）
5) Yang GJ,
Huang JL, Meng WJ, Shen M, Jiao XA. A reusable capacitive immunosensor for detection of
Salmonella spp. based on grafted ethylene diamine and self-assembled gold nanoparticle monolayers.
Anal Chim Acta.（IF 3.757） 2009, 647(2):159-166.
